Stem cells – Spheroids – Organoids – Tissues
Think in new dimensions – CERO is overcoming known limitations of static cultures.
Easy, ready-to-use protocols, standardizable workflows and autoadhesion of cells without any need for Carriers or Matrigel are just a few of the advantages.
As a result, CERO enables high yield expansion of pluripotent stem cells or long term cultivation of tissue for >20 days (Spheroids >80 or Organoids >180).
Cultivating hepatocyte spheroids in CERO considerably improves expansion, differentiation, maturation and hepatic virus infection compared to monolayer culture. Our research takes advantage from healthy cells even from long-term cultures in CERO. Moreover, we are now able to perform 3D long-term culture of human tissue specimen in CERO - a paradigm shift.
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Generation of HepaRG Spheroids in the CERO
Scientific Poster: "Establishment and validation of the 3D cultivation system“CERO” for hepatic spheroids to study viral hepatitis", presented at the 2018 EMBL Symposium "Organoids: Modelling Organ Development and Disease in 3D Culture"
Expansion Protocol Pluripotent Stem Cells (iPSC and ESC)
Differentiation Protocol Organoids from Pluripotent Stem Cells
Britta Gerlach, PhD
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