ITS supplement is a basal growth factor supplement used to reduce the serum requirements in reduced serum and serum free cell culture systems.

Versand auf Trockeneis (-20 °C).

ITS-E supplement is a basal growth factor supplement used to reduce the serum requirements in reduced serum and serum free cell culture systems.

Versand auf Trockeneis (-20 °C).

ITS-P supplement is a basal growth factor supplement used to reduce the serum requirements in reduced serum and serum free cell culture systems.

Versand auf Trockeneis (-20 °C).

Phytohemagglutinin(PHA-M) is a lectin extracted from red kidney bean Phaseolus vulgaris. PHA-M is the most common mitogen used for the stimulation of cell division in lymphocyte cultures. TCL071 is a sterile filtered solution of 1mg per ml PHAM in sterile tissue culture water.

Versand auf Trockeneis (-20 °C).

HAT supplement contains Hypoxanthine, Aminopterin and Thymidine and is used for the preparation of selection medium for hybridoma. The myeloma cell is deficient in enzyme hypoxanthine-guanine phosphoribosyl transferase (HGPRT) or thymidine kinase (TK), and cannot survive in selection medium containing hypoxanthine, aminopterin and thymidine. TCL072 is a sterile filtered 50X HAT medium supplement containing 680.5 mg/litre Hypoxanthine, 8.8 mg/litre Aminopterin and 193.8 mg/litre Thymidine in Phosphate Buffered Saline.

Versand auf Trockeneis (-20 °C).

The specific action of aminopterin present in the HAT system is to inhibit the de novo DNA synthesis. Hence, it needs to be eliminated once the hybrids are selected and isolated. Aminopterin is diluted by several passages in hypoxanthine-thymidine (HT) supplemented medium for about 2-3 weeks. Once the de novo nucleoside-synthesis pathway has been reinstated, HT supplementation can be discontinued.
TCL073 is a sterile filtered, cell culture tested 50X HT medium supplement containing 680.5 mg/litre Hypoxanthine and 193.8 mg/litre Thymidine in Phosphate Buffered Saline.

Versand auf Trockeneis (-20 °C).

Colcemid, also known as demecolcine, is a synthetic analog of colchicine. It binds to the tubulin protein and obstructs the spindle fibre formation. It effectively arrests cell in metaphase, allowing cell harvest and karyotyping to be performed. Colcemid is used to arrest different types of cells in metaphase like peripheral blood cells, amniotic fluid cells, fibroblasts, bone marrow cells and cells from chorion villus samples, etc. TCL074 is a sterile filtered solution of 10μg per ml of Colcemid is Hank's balanced salt solution.

Kühlware (2°C - 8°C)

Sterile solution formulated to contain 45% w/v glucose in tissue culture grade water. This solution is sterilized by autoclaving.

Net weight: TCL077-500ML: 0,5 kg
TCL077-100ML: 0,1 kg

Giemsa stain is a mixture of methylene blue, eosin, and azure B. It is specific for the phosphate groups of DNA and attaches itself to where there are high amounts of adenine thymine bonding. Giemsa stain is mainly used for staining of peripheral blood smears and specimens obtained from the bone marrow. Giemsa stain is also used in cytogenetics to stain the chromosomes and identify chromosomal aberrations. TCL083 is a sterile filtered concentrated solution of Giemsa stain.

Net weight: TCL083-500ML: 0,5 kg
TCL083-100ML: 0,1 kg

The Glutamine Synthetase Expression Medium (GSEM) with supplement is a system that supports the growth of transfected cells containing the gene for the enzyme Glutamine synthetase. TCL088 is sterile-filtered, 50X liquid Glutamine Synthetase Expression Medium Supplement.

Versand auf Trockeneis (-20 °C).

In serum supplemented cell cultures, serum serves as a major source of extracellular lipids. In serum-free cell culture systems, lipids are added in the form of lipid supplements or lipid concentrates. These are mixtures of different fatty acids, detergents and lipids that are essential for cell growth and maintenance in serum-free conditions.
TCL091 is a sterile filtered 100X lipid supplement formulated to contain chemically defined lipids and fatty acids.

Kühlware (2°C - 8°C)

Cholesterol supplement is used as an additive in serum free media. It is an important attachment factor for increased plating efficiency. It protects cells from shear stress. TCL092 is a sterile filtered 250X cholesterol supplement.

Kühlware (2°C - 8°C)

ITS-L1 supplement is a basal growth factor supplement used to reduce the serum requirements in reduced serum and serum-free cell culture systems.
Insulin is a peptide hormone responsible for uptake, utilization and storage of glucose by cells. Insulin is often used as a medium supplement for cell culture in the range of 1-10 µg/ml depending upon the cell type.

Versand auf Trockeneis (-20 °C).

ITS-D Supplement 100X Liquid

Versand auf Trockeneis (-20 °C).

Recombinant Human Insulin Solution, 5mg/ml

Kühlware (2°C - 8°C)

Colcemid, also known as demecolcine, is a synthetic analog of colchicine. It binds to the tubulin protein and obstructs the spindle fibre formation. It effectively arrests cell in metaphase, allowing cell harvest and karyotyping to be performed. Colcemid is used to arrest different types of cells in metaphase like peripheral blood cells, amniotic fluid cells, fibroblasts, bone marrow cells and cells from chorion villus samples, etc. TCL133 is a sterile filtered solution of 10μg per ml of Colcemid in Phosphate Buffered Saline.

Kühlware (2°C - 8°C)

pH Regulator for Bioreactor Sterile filtered

Net weight: 0,5 kg

Polyamine Supplement 1000X Sterile filtered

Kühlware (2°C - 8°C)

STEMin1™ Xeno-free Attachment Solution
Suitable for xeno-free culture of stem cells

Versand auf Trockeneis (-20 °C).

STEMin1™ Xeno-free Neutralizer
Suitable for xeno-free culture of stem cells

Versand auf Trockeneis (-20 °C).