CERO 3D — Bioreactor and Incubator for 3D Cell Culture
We have redesigned the CERO 3D Incubator & Bioreactor from the inside ready for future 3D cell culture workflows. It supports advanced 3D models using stem cells, organoids, spheroids and tissue biopsies - high consistency, less hands-on work and cost-effective for short- and long-term culture.
Trusted by Hundreds of Labs Worldwide
High quality brain organoids & assembloids.
Prof. Dr. L. Sevenich and A. Wolfram | University Hospital Tübingen
Scalable cultivation of iPSCs.
Dr. J. Neubauer | Managing Director at Fraunhofer Project Center for Stem Cell Process Technology
Advanced liver tissue research.
Prof. Dr. A. Nüssler and Prof. Dr. S. Ehnert | Siegfried Weller Institute Tübingen
Your Challenges in 3D Cell Culture
Differentiation of pluripotent stem cells results in heterogeneous cell populations.
Long-term culture of organoids leads to loss of structure and metabolic activity.
Time consuming manual maintenance like media changes.
High costs for consumables and substrates.
Solvedby CERO 3D Incubator and Bioreactor
Increased cell vitality and homogeneity of cell populations
Cost-efficient cultivation with less media and no embedding substrates needed
Ideal for short- and long-term culture
Special culture vessels ensure minimal hands-on time for media change
Video above: Beating cardiac bodies from iPSC-derived cardiomyocytes, differentiated and cultivated using CERO 3D. (Max-Planck-Institute for Heart and Lung Research)
CERO 3D - Key Facts
Enhanced Cell Viability and Vitality
CERO 3D’s low-shear bi-directional rotation minimizes cell stress and damage, promoting long-term culture, survival, and differentiation without compromising cell health.
Flexible & Scalable
CERO 3D supports a wide range of cell types—including stem cells, tumor cells, and even tissue biopsies—offering scalable 3D culture in four individually controlled tubes, all in a compact benchtop footprint.
Automated Environmental Control
Precise control of CO₂, temperature, and pH ensures optimal conditions for growing and differentiating organoids, tissue biopsies, and other 3D structures.
Budget-friendly & Easy handling
Intuitive operation with minimal hands-on time—quick 4-minute media changes, reduced media consumption, and no need for embedding substrates.
Extended Culturing for Long-Term Studies
Designed for long-term studies, CERO 3D supports cultures from days to months—ideal for regenerative medicine, cancer research, and viral studies.
Advanced 3D Cell Culture
Achieve more realistic cell behavior and interactions with 3D culture—enhancing accuracy in cancer research, tissue modeling, and regenerative medicine.
Trusted by Experts
Applications
Cerebral Organoids and Brain Cancer Assembloids
A report from the Sevenich lab on brain organoids and tumour assembloids.
Culturing pluripotent stem cells in suspension, without embedding substrates.
Spheroid models in hepatitis virus research.
Keeping liver tissue in culture over longer periods.
Cardiac tissue models for research and safety testing.
Organoids used as a tumour tissue model.
Immunotherapy research on three-dimensional models.
Studies in which CERO 3D was used.
FAQs
Request a CERO 3D demo
Tell us what you would like to see and we will arrange a demonstration in your lab or online.
CERO 3D is a high-performance incubator and bioreactor designed for 3D cell culture. It enables the cultivation of spheroids, organoids, and tissue-like structures under physiological conditions.
CERO 3D supports a wide range of cell types, including stem cells, cancer cells, primary cells, and cell lines used in organoid and spheroid models. It is suitable for both adherent and suspension cell cultures.
Unlike static culture methods, CERO 3D provides dynamic, rotation-based cultivation, preventing shear stress while ensuring optimal nutrient and oxygen exchange. This results in more uniform and physiologically relevant 3D cell structures.
Yes, CERO 3D supports co-culture systems, allowing multiple cell types to grow together in a controlled environment. This makes it ideal for disease models and tissue engineering.
Yes, CERO 3D is designed for seamless integration with standard lab equipment. It is compatible with microscopy, flow cytometry, and other analytical techniques, allowing easy downstream analysis of 3D cultures.
Rotation-based 3D culture system for natural cell growth Controlled temperature, oxygen, and CO₂ levels Compatible with a variety of culture media and scaffolds Supports long-term cultures Scalable design for different research needs
CERO 3D is widely used in: Cancer research Drug screening and toxicity testing Regenerative medicine and tissue engineering Neuroscience (brain organoids) Immunology and immune-oncology studies
Yes, CERO 3D features an intuitive user interface and pre-installed protocols cultivating different cell types, making it easy to set up and maintain complex 3D cultures without extensive training.
Our experts and OLS service team provide prompt troubleshooting, technical assistance, and maintenance support to ensure optimal performance of your system. Contact us for fast and reliable solutions.