Normal collagenase activity with a minimum of proteolytic activity. Especially recommended for breast tissue.
Store at 2 – 8° C.


Storage Temperature: +2°C - +8°C

Equivalent/Alternative to Biochrom:
100 mg: Biochrom Cat.-no. C 3-28
1 g: Biochrom Cat.-no. C 3-22

COLLAGENASE TYPE I:
Natural balance of enzyme activity. Recommended for cell preparation from epithelial lung tissue of the urprarenal gland and adipose tissue.
STORAGE:
Store at 2 – 8° C.


Storage Temperature: +2°C - +8°C

Equivalent/Alternative to Biochrom:
100 mg: Biochrom Cat.-no. C 1-28

APPLICATION:
For dissociation of tissue and cell monolayer cultures.
SHELF LIFE:
Solution 24 months, powder 36 months. The shelf life commences with date of production.
STORAGE:
Solution at -20° C, powder at 2 - 8° C.
OTHER SIZES AND CUSTOM FORMULATION:
Please ask for other sizes or special formulations of trypsin; in most cases we can provide a solution. Our trypsin is tested negative for mycoplasma and PPV.


Storage Temperature: -20°C

Equivalent/Alternative to Biochrom:
100 ml: Biochrom Cat.-no. L 2153

With especially high activity of clostripain and trypsin. Recommended for cell preparation from liver tissue, bone tissue, cardiac tissue, thyroid gland tissue and salivary gland tissue.
Store at 2 – 8° C.


Storage Temperature: +2°C - +8°C

Equivalent/Alternative to Biochrom:
100 mg: Biochrom Cat.-no. C 2-28
1 g: Biochrom Cat.-no. C 2-22

Accutase ® is the gentle replacement for Trypsin in all adherent cell cultures and especially when working with sensitive cell types. Accutase ® is a non-mammalian, non-bacterial ready-to-use solution.
PAN-Biotech produces and distributes Accutase ® under license of the patentee Innovative Cell Technologies. Exclusive and for the whole of Europe.
COMPOSITION:
Accutase ® enzymes (activity > 500 U/ml) in DPBS w/o Ca/Mg with 0.5 mM EDTA and phenol red.
SUITABILITY:
Accutase ® can be used as a direct replacement of Trypsin for cell dissociation. Accutase ® is a ready-to-use cell detachment solution made of collagenolytic and proteolytic enzymes. It is used for routine detachment of adherent cells from tissue culture plates and flasks. A multitude of cell types has been successfully sub-cultured with Accutase ® , including fibroblasts, endothelial, vascular smooth muscle cells, as well as hepatocytes, embryonal stem cells, and many immortalized cell lines such as adherent CHO and BHK cells, HEK 293, L929, HeLa, 3T3, and others. Accutase ® is free of mammalian or bacterial products.
Gentle detachment of cells for:
- analysis of cell surface markers,
- transfection procedures,
- migration or proliferation assays,
- flow cytometry,
- and routine cell passage.
SPECIAL ADVANTAGES:
- Ready-to-use solution
- Gentle and effective cell detachment
- Lower contamination risk
- No neutralization required
- Protection of surface epitopes
- Maximum cell viability and enhanced plating efficiency
- Alternative to Trypsin

Storage Temperature: -20°C

Accumax can be used as a direct replacement of trypsin for cell detachment or collagenase for cell dissociation or as a anti-clumping agent for cell culture, flow cytometry, and FACS.
SPECIAL ADVANTAGES:
- Ready-to-use solution
- Neutralizing of the Accumax enzymes is not required for routine cell culture passaging.
- The product is active at room temperature, no pre-warming required or recommended.
- Preserves most epitopes for subsequent flow cytometry analysis.
- Gentle detachment of cells for analysis of cell surface markers, transfection procedures, migration or proliferation assays, flow cytometry, and routine cell passage.

Storage Temperature: -20°C

In many cases the isolation of cells is the first step for gene expression studies or in diagnostic procedures. Besides biological separation techniques physical separation methods are most commonly used. These methods use physical differences such as size and weight of the particles to be separated. For this purpose so-called separating solutions (= centrifugation media) are used.
THESE MEDIA MUST MEET THE FOLLOWING CRITERIA::
- They must be able to form a density gradient over the desired range
- The desired pH value and osmolality must be easy to adjust
- The solutions must not be too viscous at high density
- They must not cause functional or morphological changes to biological materials
- They must not penetrate biological membranes
Our Pancoll separating solutions contain a polysaccharide with a molecular weight of 400,000 daltons; this hydrophilic polymer allows for production of aqueous solutions for cell separation with a density of up to 1.2 g/ml. PAN-Biotech offers a variety of ready-to-use products with a density of 1.063 g/ml up to 1.091 g/ml for a very wide range of cell separation applications.
STORAGE:
2° C to ambient temperature "Protect from light!“
When properly stored, separating solutions are stable for at least 36 months. The storage period starts with the manufacturing date.

Storage Temperature: +2°C - RT

Cryopan III is a serum-free freezing medium without DMSO, suitable for the cryopreservation of various cell types.
KEY FEATURES::
- Versatility: Cryopan III is a specially developed cryopreservation medium designed for freezing various human and animal cells. Its optimized formulation is suitable for preserving both adherent and suspension cells. It is ideal for the long-term storage of established cell lines, primary cells and tissue samples such as umbilical cord tissue.
- Composition: Cryopan III consists of a chemically defined and optimized mixture of salts, sugar and additional antifreeze-substances. It contains no animal or human components and is free of DMSO.
- Special Advantages: The formulation without DMSO is particularly well-suited for primary cells and other applications in which the cytotoxic side-effects associated with DMSO are not desired. The serum-free freezing medium is particularly well-suited for preserving serum-free cultured cells. The optimized composition guarantees high cell viability post-thawing.

Storage Temperature: -20°C

Cryopan II is a serum-free and protein-free cryopreservation medium for the cryopreservation of a broad range of cell types. It ensures optimal cell preservation without the interference of human or animal components.
KEY FEATURES:
- Versatility : Suitable for freezing both adherent and suspension cells, Cryopan II as specially developed cryopreservation medium can be used for various cell types and culture conditions.
- Composition : A chemically defined formulation of salts, sugar, 10% DMSO, and additional antifreeze-substances, free from animal or human components.
- Special Advantages : Its serum-free formulation is particularly well-suited for preserving serum-free cultured cells. The optimized composition guarantees high cell viability post-thawing.
APPLICATIONS:
- Stem Cell Research and Therapy
- Tissue Engineering
- Cellular Therapies
- Biobanking
- Gene Therapy
- Research and Development
- Vaccine Production

Storage Temperature: -20°C

Cryopan I is a serum-free freezing medium for the cryoconservation of cells in a nitrogen storage tank or an ultra-low temperature freezer (< -150 °C). It contains 10 % DMSO.
FREEZING PROCEDURE:
- Refrigerate freezing medium, culture medium and freezing tubes!
- Trypsinize cells, transfer the cells into the culture medium and centrifuge. Discard the supernatant and resuspend cells in culture medium.
- Cell count should be adjusted to 1 - 5 x 10 6 /ml. The cells should be carefully resuspended to avoid clustering.
- Spin down and resuspend the cells in an appropriate volume of cool freezing medium by pipetting only once or twice. Distribute 1 ml cell suspension per freezing tube.
- To achieve a defined freezing rate of about 1 °C per minute, manual freezing devices or computer controlled freezing may be used.
- Alternatively, put the tubes for 15 minuts into a refrigerator, so that the freezing medium can penetrate into the cells. After this step freeze the tubes at -20 °C for 2 hours. Put the cells into the vapour phase of liquid nitrogen over night.
- Then transfer the freezing tubes into liquid nitrogen.

Storage Temperature: -20°C

Trypsin is a serine protease derived from porcine pancreas. Ethylenediaminetetraacetic acid (EDTA), a chelating agent is often added to enhance enzymatic activity of trypsin solution. This product contains 0.25% Trypsin and 0.2% EDTA in Dulbecco’s phosphate buffered saline.

Shipped on dry ice (-20 °C).

Trypsin is a serine protease derived from porcine pancreas. This product is 0.25% Trypsin in Dulbecco’s phosphate buffered saline.

Net weight: TCL006-500ML: 0,5 kg
TCL006-5x100ML: 0,5 kg
TCL006-100ML: 0,1 kg

Shipped on dry ice (-20 °C).

Trypsin-EDTA solution contains 0.25% Trypsin and 0.02% EDTA in Dulbecco’s phosphate buffered saline.

Net weight: TCL007-500ML: 0,5 kg
TCL007-100ML: 0,1 kg

Shipped on dry ice (-20 °C).

Trypsin is most commonly used for dissociation and disaggregation of adherent cells. It is 0.25% Trypsin in Hank’s balanced salt solution.


Net weight: TCL008-500ML: 0,5 kg
TCL008-100ML: 0,1 kg

Shipped on dry ice (-20 °C).

Trypsin is a serine protease derived from porcine pancreas. This product is 0.1% Trypsin in Dulbecco’s phosphate buffered saline.

Net weight: TCL011-500ML: 0, 5 kg
TCL011-5x100ML: 0,5 kg
TCL011-100ML: 0,1 kg

Shipped on dry ice (-20 °C).

Trypsin is a serine protease derived from porcine pancreas. Ethylenediaminetetraacetic acid (EDTA), a chelating agent is often added to enhance enzymatic activity of trypsin solutionThis product contains 0.25% Trypsin and 0.001% EDTA in Dulbecco’s phosphate buffered saline.

Net weight: TCL014-500ML: 0,5 kg
TCL014-5x100ML: 0,5 kg
TCL014-100ML: 0,1 kg

This product contains 0.25% Trypsin, 0.5% PVP, 0.02% EDTA and 0.05% Glucose in Dulbecco’s phosphate buffered saline.

Net weight: TCL022-500ML: 0,5 kg
TCL022-5X100ML: 0,5 kg
TCL022-100ML: 0,1 kg

Shipped on dry ice (-20 °C).

ZymeFree™ works as quick as trypsin on most cell lines. It holds the advantage over enzyme based dissociation solutions as it does not cause cytotoxicity, even after cells are exposed to it for prolonged period.

Net weight: TCL028-2X500ML: 1 kg
TCL028-100ML: 0,1 kg

refrigerated goods (2°C - 8°C)

Trypsin is a serine protease derived from porcine pancreas. Ethylenediaminetetraacetic acid (EDTA), a chelating agent is often added to enhance enzymatic activity of trypsin solution. This product contains 0.1% Trypsin, 0.02% EDTA, 0.05% Glucose and Phenol red in Dulbecco’s phosphate buffered saline.

Net weight: TCL031-500ML: 0,5 kg
TCL031-5X100ML: 0,5 kg
TCL031-100ML: 0,1 kg

Shipped on dry ice (-20 °C).

Trypsin is a serine protease derived from porcine pancreas. Ethylenediaminetetraacetic acid (EDTA), a chelating agent is often added to enhance enzymatic activity of trypsin solution. This product contains 0.05% Trypsin and 0.02% EDTA and phenol red in Hank’s balanced salt solution.

Net weight: TCL033-500ML: 0,5 kg
TCL033-5X100ML: 0,5 kg
TCL033-100ML: 0,1 kg

Shipped on dry ice (-20 °C).