DMSO (Dimethylsulfoxide) is a colourless organic solvent which enters the cell and distributes inside the cell to prevent the formation of damaging ice crystals during the freezing procedure.

Storage Temperature: RT

DMSO (Dimethylsulfoxide) is a colourless organic solvent which enters the cell and distributes inside the cell to prevent the formation of damaging ice crystals during the freezing procedure.

Storage Temperature: RT

Our freezing medium is recommended for the cryoconservation of cells. The medium is based on DMEM, supplemented with a mix of fetal bovine serum and DMSO. This composition guarantees a high survival rate and excellent cell growth after thawing.

Storage Temperature: -20°C

Cryopan III is a serum-free freezing medium without DMSO, suitable for the cryopreservation of various cell types.
KEY FEATURES::
- Versatility: Cryopan III is a specially developed cryopreservation medium designed for freezing various human and animal cells. Its optimized formulation is suitable for preserving both adherent and suspension cells. It is ideal for the long-term storage of established cell lines, primary cells and tissue samples such as umbilical cord tissue.
- Composition: Cryopan III consists of a chemically defined and optimized mixture of salts, sugar and additional antifreeze-substances. It contains no animal or human components and is free of DMSO.
- Special Advantages: The formulation without DMSO is particularly well-suited for primary cells and other applications in which the cytotoxic side-effects associated with DMSO are not desired. The serum-free freezing medium is particularly well-suited for preserving serum-free cultured cells. The optimized composition guarantees high cell viability post-thawing.

Storage Temperature: -20°C

Cryopan II is a serum-free and protein-free cryopreservation medium for the cryopreservation of a broad range of cell types. It ensures optimal cell preservation without the interference of human or animal components.
KEY FEATURES:
- Versatility : Suitable for freezing both adherent and suspension cells, Cryopan II as specially developed cryopreservation medium can be used for various cell types and culture conditions.
- Composition : A chemically defined formulation of salts, sugar, 10% DMSO, and additional antifreeze-substances, free from animal or human components.
- Special Advantages : Its serum-free formulation is particularly well-suited for preserving serum-free cultured cells. The optimized composition guarantees high cell viability post-thawing.
APPLICATIONS:
- Stem Cell Research and Therapy
- Tissue Engineering
- Cellular Therapies
- Biobanking
- Gene Therapy
- Research and Development
- Vaccine Production

Storage Temperature: -20°C

Cryopan I is a serum-free freezing medium for the cryoconservation of cells in a nitrogen storage tank or an ultra-low temperature freezer (< -150 °C). It contains 10 % DMSO.
FREEZING PROCEDURE:
- Refrigerate freezing medium, culture medium and freezing tubes!
- Trypsinize cells, transfer the cells into the culture medium and centrifuge. Discard the supernatant and resuspend cells in culture medium.
- Cell count should be adjusted to 1 - 5 x 10 6 /ml. The cells should be carefully resuspended to avoid clustering.
- Spin down and resuspend the cells in an appropriate volume of cool freezing medium by pipetting only once or twice. Distribute 1 ml cell suspension per freezing tube.
- To achieve a defined freezing rate of about 1 °C per minute, manual freezing devices or computer controlled freezing may be used.
- Alternatively, put the tubes for 15 minuts into a refrigerator, so that the freezing medium can penetrate into the cells. After this step freeze the tubes at -20 °C for 2 hours. Put the cells into the vapour phase of liquid nitrogen over night.
- Then transfer the freezing tubes into liquid nitrogen.

Storage Temperature: -20°C

Cell Freezing Media are complete, ready to use reagents designed to protect and preserve cells during frozen storage. Cell Freezing Medium with DMSO is fully supplemented formulation prepared in Dulbecco’s Modified Eagle Medium. It contains 10% DMSO and foetal bovine serum. DMSO acts as a cryoprotectant and prevents formation of ice crystals and prevents cell damage. It is ready to use and does not require further addition of any other reagents. This cryopreservation medium can be used for hardy cell lines that are less susceptible to freezing damage.

Net weight: 0,05 kg

Shipped on dry ice (-20 °C).

Cell Freezing Medium with Glycerol is fully supplemented formulation prepared in Dulbecco’s Modified Eagle Medium. It contains 10% Glycerol and foetal bovine serum. Glycerol acts a cryoprotectant as it prevents formation of ice crystals and prevents cell damage. It is ready to use and does not require further addition of any other reagents. This cryopreservation medium can be used for hardy cell lines that are less susceptible to freezing damage.

Net weight: 0,05 kg

Shipped on dry ice (-20 °C).

Cell Freezing Media are complete, ready to use reagents designed to protect and preserve cells during frozen storage. Cell Freezing Medium with DMSO is fully supplemented formulation prepared in Dulbecco’s Modified Eagle Medium. It contains 10% DMSO and foetal bovine serum. DMSO acts as a cryoprotectant and prevents formation of ice crystals and prevents cell damage. It is ready to use and does not require further addition of any other reagents. This cryopreservation medium can be used for hardy cell lines that are less susceptible to freezing damage.

Net weight: 0,05 kg

Shipped on dry ice (-20 °C).

Cell Freezing Medium with Glycerol is fully supplemented formulation prepared in Dulbecco’s Modified Eagle Medium. It contains 10% Glycerol and fetal bovine serum. Glycerol acts as a cryoprotectant as it prevents formation of ice crystals and prevents cell damage. It does not contain phenol red. It is ready to use and does not require further addition of any other reagents. This cryopreservation medium can be used for hardy cell lines that are less susceptible to freezing damage.

Net weight: 0,05 kg

Shipped on dry ice (-20 °C).

Cell Freezing Media are complete, ready to use reagents designed to protect and preserve cells during frozen storage. These media are a convenient and cost effective alternative to in-house freezing media and can be used for a wide variety of mammalian cells. TCL093 contains 10% DMSO and higher concentration of fetal bovine serum. It is ready to use and does not require further addition of any other reagents. This cryopreservation medium can be used for sensitive cell lines that are very susceptible to freezing damage.

Net weight: 0,05 kg

Shipped on dry ice (-20 °C).

It is chemically defined serum free cell freezing medium designed to freeze the cells grown in serum free and animal component free conditions. This medium ensures high cell viability upon revival from cryo-storage and can also be used for cryopreservation of stem cells and primary cells. This medium is a proprietary formulation.

Net weight: 0,05 kg

Shipped on dry ice (-20 °C).

It is Hybridoma Cell Freezing Medium formulated to contain 10% DMSO and fetal bovine serum. The medium does not contain antibiotics. DMSO acts as a cryoprotectant and prevents formation of ice crystals and prevents cell damage. It is ready to use and does not require further addition of any other reagents. This medium is optimized for cryopreservation of sensitive hybridoma cell lines.

Net weight: 0,05 kg

Shipped on dry ice (-20 °C).