APPLICATION:
For dissociation of tissue and cell monolayer cultures.
SHELF LIFE:
Solution 24 months, powder 36 months. The shelf life commences with date of production.
STORAGE:
Solution at -20° C, powder at 2 - 8° C.
OTHER SIZES AND CUSTOM FORMULATION:
Please ask for other sizes or special formulations of trypsin; in most cases we can provide a solution. Our trypsin is tested negative for mycoplasma and PPV.


Storage Temperature: -20°C

Equivalent/Alternative to Biochrom:
100 ml: Biochrom Cat.-no. L 2143

Selected low tryptic activity at high collagenase activity and normal clostripain level. Recommended for cell preparation from the pancreatic island. Store at 2 – 8° C.


Storage Temperature: +2°C - +8°C

Equivalent/Alternative to Biochrom:
100 mg: Biochrom Cat.-no. C 4-28
1 g: Biochrom Cat.-no. C 4-22

Normal collagenase activity with a minimum of proteolytic activity. Especially recommended for breast tissue.
Store at 2 – 8° C.


Storage Temperature: +2°C - +8°C

Equivalent/Alternative to Biochrom:
100 mg: Biochrom Cat.-no. C 3-28
1 g: Biochrom Cat.-no. C 3-22

COLLAGENASE TYPE I:
Natural balance of enzyme activity. Recommended for cell preparation from epithelial lung tissue of the urprarenal gland and adipose tissue.
STORAGE:
Store at 2 – 8° C.


Storage Temperature: +2°C - +8°C

Equivalent/Alternative to Biochrom:
100 mg: Biochrom Cat.-no. C 1-28

APPLICATION:
For dissociation of tissue and cell monolayer cultures.
SHELF LIFE:
Solution 24 months, powder 36 months. The shelf life commences with date of production.
STORAGE:
Solution at -20° C, powder at 2 - 8° C.
OTHER SIZES AND CUSTOM FORMULATION:
Please ask for other sizes or special formulations of trypsin; in most cases we can provide a solution. Our trypsin is tested negative for mycoplasma and PPV.


Storage Temperature: -20°C

Equivalent/Alternative to Biochrom:
100 ml: Biochrom Cat.-no. L 2153

With especially high activity of clostripain and trypsin. Recommended for cell preparation from liver tissue, bone tissue, cardiac tissue, thyroid gland tissue and salivary gland tissue.
Store at 2 – 8° C.


Storage Temperature: +2°C - +8°C

Equivalent/Alternative to Biochrom:
100 mg: Biochrom Cat.-no. C 2-28
1 g: Biochrom Cat.-no. C 2-22

Accutase ® is the gentle replacement for Trypsin in all adherent cell cultures and especially when working with sensitive cell types. Accutase ® is a non-mammalian, non-bacterial ready-to-use solution.
PAN-Biotech produces and distributes Accutase ® under license of the patentee Innovative Cell Technologies. Exclusive and for the whole of Europe.
COMPOSITION:
Accutase ® enzymes (activity > 500 U/ml) in DPBS w/o Ca/Mg with 0.5 mM EDTA and phenol red.
SUITABILITY:
Accutase ® can be used as a direct replacement of Trypsin for cell dissociation. Accutase ® is a ready-to-use cell detachment solution made of collagenolytic and proteolytic enzymes. It is used for routine detachment of adherent cells from tissue culture plates and flasks. A multitude of cell types has been successfully sub-cultured with Accutase ® , including fibroblasts, endothelial, vascular smooth muscle cells, as well as hepatocytes, embryonal stem cells, and many immortalized cell lines such as adherent CHO and BHK cells, HEK 293, L929, HeLa, 3T3, and others. Accutase ® is free of mammalian or bacterial products.
Gentle detachment of cells for:
- analysis of cell surface markers,
- transfection procedures,
- migration or proliferation assays,
- flow cytometry,
- and routine cell passage.
SPECIAL ADVANTAGES:
- Ready-to-use solution
- Gentle and effective cell detachment
- Lower contamination risk
- No neutralization required
- Protection of surface epitopes
- Maximum cell viability and enhanced plating efficiency
- Alternative to Trypsin

Storage Temperature: -20°C

Accumax can be used as a direct replacement of trypsin for cell detachment or collagenase for cell dissociation or as a anti-clumping agent for cell culture, flow cytometry, and FACS.
SPECIAL ADVANTAGES:
- Ready-to-use solution
- Neutralizing of the Accumax enzymes is not required for routine cell culture passaging.
- The product is active at room temperature, no pre-warming required or recommended.
- Preserves most epitopes for subsequent flow cytometry analysis.
- Gentle detachment of cells for analysis of cell surface markers, transfection procedures, migration or proliferation assays, flow cytometry, and routine cell passage.

Storage Temperature: -20°C

Trypsin is a serine protease derived from porcine pancreas. Ethylenediaminetetraacetic acid (EDTA), a chelating agent is often added to enhance enzymatic activity of trypsin solution. This product contains 0.25% Trypsin and 0.2% EDTA in Dulbecco’s phosphate buffered saline.

Shipped on dry ice (-20 °C).

Trypsin is a serine protease derived from porcine pancreas. This product is 0.25% Trypsin in Dulbecco’s phosphate buffered saline.

Net weight: TCL006-500ML: 0,5 kg
TCL006-5x100ML: 0,5 kg
TCL006-100ML: 0,1 kg

Shipped on dry ice (-20 °C).

Trypsin-EDTA solution contains 0.25% Trypsin and 0.02% EDTA in Dulbecco’s phosphate buffered saline.

Net weight: TCL007-500ML: 0,5 kg
TCL007-100ML: 0,1 kg

Shipped on dry ice (-20 °C).

Trypsin is most commonly used for dissociation and disaggregation of adherent cells. It is 0.25% Trypsin in Hank’s balanced salt solution.


Net weight: TCL008-500ML: 0,5 kg
TCL008-100ML: 0,1 kg

Shipped on dry ice (-20 °C).

Trypsin is a serine protease derived from porcine pancreas. This product is 0.1% Trypsin in Dulbecco’s phosphate buffered saline.

Net weight: TCL011-500ML: 0, 5 kg
TCL011-5x100ML: 0,5 kg
TCL011-100ML: 0,1 kg

Shipped on dry ice (-20 °C).

Trypsin is a serine protease derived from porcine pancreas. Ethylenediaminetetraacetic acid (EDTA), a chelating agent is often added to enhance enzymatic activity of trypsin solutionThis product contains 0.25% Trypsin and 0.001% EDTA in Dulbecco’s phosphate buffered saline.

Net weight: TCL014-500ML: 0,5 kg
TCL014-5x100ML: 0,5 kg
TCL014-100ML: 0,1 kg

This product contains 0.25% Trypsin, 0.5% PVP, 0.02% EDTA and 0.05% Glucose in Dulbecco’s phosphate buffered saline.

Net weight: TCL022-500ML: 0,5 kg
TCL022-5X100ML: 0,5 kg
TCL022-100ML: 0,1 kg

Shipped on dry ice (-20 °C).

ZymeFree™ works as quick as trypsin on most cell lines. It holds the advantage over enzyme based dissociation solutions as it does not cause cytotoxicity, even after cells are exposed to it for prolonged period.

Net weight: TCL028-2X500ML: 1 kg
TCL028-100ML: 0,1 kg

refrigerated goods (2°C - 8°C)

Trypsin is a serine protease derived from porcine pancreas. Ethylenediaminetetraacetic acid (EDTA), a chelating agent is often added to enhance enzymatic activity of trypsin solution. This product contains 0.1% Trypsin, 0.02% EDTA, 0.05% Glucose and Phenol red in Dulbecco’s phosphate buffered saline.

Net weight: TCL031-500ML: 0,5 kg
TCL031-5X100ML: 0,5 kg
TCL031-100ML: 0,1 kg

Shipped on dry ice (-20 °C).

Trypsin is a serine protease derived from porcine pancreas. Ethylenediaminetetraacetic acid (EDTA), a chelating agent is often added to enhance enzymatic activity of trypsin solution. This product contains 0.05% Trypsin and 0.02% EDTA and phenol red in Hank’s balanced salt solution.

Net weight: TCL033-500ML: 0,5 kg
TCL033-5X100ML: 0,5 kg
TCL033-100ML: 0,1 kg

Shipped on dry ice (-20 °C).

Trypsin is a serine protease derived from porcine pancreas. Ethylenediaminetetraacetic acid (EDTA), a chelating agent is often added to enhance enzymatic activity of trypsin solution. This product contains 0.5% Trypsin and 0.2% EDTA in 0.85% normal saline. It does not contain Phenol red.

Net weight: TCL034-500ML: 0,5 kg
TCL034-100ML: 0,1 kg

Shipped on dry ice (-20 °C).

Trypsin is a serine protease derived from porcine pancreas. Ethylenediaminetetraacetic acid (EDTA), a chelating agent is often added to enhance enzymatic activity of trypsin solution. This product contains 0.25% Trypsin and 0.1% EDTA in Hank’s balanced salt solution. It does not contain Phenol red.

Net weight: TCL042-500ML: 0,5 kg
TCL042-5X100ML: 0,5 kg
TCL042-100ML: 0,1 kg

Shipped on dry ice (-20 °C).