[AL242] Basal Medium developed by Harry Eagle is a combination of essential nutrients in appropriate concentrations for monolayer cultivation of a wide variety of normal and transformed cells. Basal Medium Eagle (BME) is with Earle's salts and Sodium bicarbonate and without L-Glutamine.

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[AL243A] Dulbecco's Modified Eagle Medium /Nutrient Mixture F12 Ham (DMEM/F12, 1:1 mixture) was originally formulated for rat neuroblastoma cells and MDCK cells.AL243A is DMEM/Nutrient Mixture F-12 Ham with LGlutamine, 2mg/L Riboflavin, 55mg/L Sodium pyruvate and Sodium bicarbonate. It does not contain trace elements, HEPES buffer and Pyridoxal hydrochloride.

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[AL247A] Leibovitz’s Medium was specifically designed to grow cells in a CO2 free atmosphere. The medium can be used to grow human tumor cells and embryonic cells and also established cell lines like HeLa and Hep-2. The medium is frequently used in diagnostic virology where tissue cell lines or strains need to be grown in closed systems. Leibovitz’s medium obviates the need of frequent medium change. AL247A is 2X concentrate with L-glutamine.

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[AL249A] Cytogenetic studies include metaphase and pro-metaphase studies carried out on lymphocytes to detect chromosomal aberrations associated with structural and numerical abnormalities. Lymphocytes come from normal peripheral blood and are mitotically inactive, hence have to be stimulated with a mitogen such as Phytohemagglutinin M(PHA-M) or Phytohemagglutinin P(PHA-P). AL249A is composed of a basal medium RPMI 1640 and supplemented with L-glutamine, FBS, PHA-P, penicillin, streptomycin and sodium bicarbonate. It is a complete medium and does not require supplementation with any additional components.

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[AL250] HiPer® Dulbecco's Modified Eagle Medium is high Glucose basal medium optimized for growth of undifferentiated embryonic and mesenchymal stem cells. It contains 4.5gms Glucose per litre, Sodium pyruvate and Sodium bicarbonate. It does not contain L-Glutamine.

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[AL250A] HiPer® Dulbecco's Modified Eagle Medium is high Glucose basal medium optimized for growth of undifferentiated embryonic and mesenchymal stem cells. It contains 4.5gms Glucose per litre, L-Glutamine, Sodium pyruvate and Sodium bicarbonate.

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[AL251] DMEM is a modification of Basal Medium Eagle (BME) that contains four fold concentration of amino acids and vitamins.AL251 is Dulbecco's Modified Eagle Medium with
4.5gms glucose per litre, sodium pyruvate, D-Valine and sodium bicarbonate. It does not contain Lglutamine and L-Valine.

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[AL253A] Minimum Essential Medium(MEM) was developed by Harry Eagle to meet the specific nutritional requirements of certain subtypes of HeLa cells and normal mammalian fibroblasts. By eliminating calcium, it can facilitate growth of cells in suspension cultures. AL253A is alpha modification of Minimum Essential Medium Eagle with L-glutamine, deoxyribonucleoside, ribonucleoside and sodium bicarbonate. It does not contain phenol red.

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[AL255G] HiGlutaXL™ medium contains the stabilised dipeptide form of L-glutamine, L-alanyl-L-glutamine. It offers several advantages over the conventional glutamine containing media. AL255G is alpha modification of HiGlutaXL™ Minimum Essential Medium Eagle with L-alanyl-Lglutamine, sodium bicarbonate and non-essential amino acid. It is devoid of deoxyribonucleosides, ribonucleosides and phenol red.

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[AL258A] Medium 199 was the first nutritionally defined medium developed by Morgan, Morton, and Parker in 1950 for nutritional studies on primary chick embryo fibroblasts in the absence of any additives. Media 199 are presently used for the maintenance of non-transformed cells, vaccine and virus production and primary explants of epithelial cells. AL258A is 2X concentrate with Earle's salts, L-glutamine and sodium bicarbonate.

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[AL260A] Minimum Essential Medium(MEM) was developed by Harry Eagle to meet the specific nutritional requirements of certain subtypes of HeLa cells and normal mammalian fibroblasts. By eliminating calcium, it can facilitate growth of cells in suspension cultures. AL260A contains Earle's salt, 25mM HEPES buffer, non-essential amino acid, L-glutamine and sodium bicarbonate.

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[AL264A] DMEM is a modification of Basal Medium Eagle (BME) that contains four fold concentration of amino acids and vitamins. Dulbecco's Modified Eagle Medium is with 4.5gm glucose per litre, L-glutamine, sodium pyruvate and sodium bicarbonate. It does not contain phenol red.

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[AL267] Medium 199 was the first nutritionally defined medium developed by Morgan, Morton, and Parker in 1950 for nutritional studies on primary chick embryo fibroblasts in the absence of any additives. Media 199 are presently used for the maintenance of non-transformed cells, vaccine and virus production and primary explants of epithelial cells. AL267 contains Hank's salts and sodium bicarbonate. It does not contain L-glutamine.

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[AL269A] Dulbecco's Modified Eagle Medium (DMEM) is one of the most widely used modification of Eagle's medium. It contains L-glutamine and sodium bicarbonate. It does not contain Glucose and sodium pyruvate.

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[AL271S] Kaighn’s modification of Ham’s F-12 is a complex formulation of F-12 with increased amounts of amino acids and puruvate. Salts used in this formulation are as given by Konisberg. AL271S contains sodium bicarbonate and does not contain L-glutamine.

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[AL273G] HiGlutaXL™ Dulbecco’s Modified Eagle Medium/ Nutrient Mixture F-12 Ham (DMEM / F12, 1:1 Mixture) w/ L-Alanyl-L-Glutamine and 1.2gms per litre Sodium bicarbonate w/o HEPES buffer and Trace elements

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[AL281A] DMEM is a modification of Basal Medium Eagle (BME) that contains four fold concentration of amino acids and vitamins. AL281A is DMEM with L-glutamine and 4.5gms per
litre glucose. It does not contain sodium pyruvate and sodium bicarbonate.

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[AL285A] Minimum Essential Medium(MEM) was developed by Harry Eagle to meet the specific nutritional requirements of certain subtypes of HeLa cells and normal mammalian fibroblasts. By eliminating calcium, it can facilitate growth of cells in suspension cultures. AL285A is alpha modification of Minimum Essential Medium Eagle with L-glutamine and sodium bicarbonate. It does not contain sodium pyruvate, ribonucleoside and deoxyribonucleoside.

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[AL290A] Ham's Nutrient Mixture F10 was designed for clonal growth of CHO cells and chick embryo cells under serum free conditions. It is now widely used for culturing a variety of cells including human diploid cells and white blood cells for chromosomal analysis and primary explants of rat, rabbit and chicken tissues. AL290A contains L-glutamine, 10mM HEPES buffer and sodium bicarbonate. It does not contain phenol red.

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[AL291] Dulbecco’s Modified Eagle Medium (DMEM), High Glucose, 10X w/ 4.5gms Glucose per litre, Sodium pyruvate and Sodium bicarbonate w/o L-Glutamine

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