[TCL065] ITS-E supplement is a basal growth factor supplement used to reduce the serum requirements in reduced serum and serum free cell culture systems.

Net weight: 0,01 kg

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[TCL066] ITS-P supplement is a basal growth factor supplement used to reduce the serum requirements in reduced serum and serum free cell culture systems.

Net weight: 0,01 kg

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[TCL068] Trypsin Inhibitor is soyabean trypsin inhibitor solution formulated to contain 1mg per ml trypsin inhibitor in Dulbecco's Phosphate Buffered Saline.

Net weight: 0,1 kg

refrigerated goods (2°C - 8°C)

[TCL070] This product is 2.5% Trypsin, 0.2% EDTA in 0.85% normal saline.

Net weight: TCL070-500ML: 0,5 kg
TCL070-100ML: 0,1 kg

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[TCL071] Phytohemagglutinin(PHA-M) is a lectin extracted from red kidney bean Phaseolus vulgaris. PHA-M is the most common mitogen used for the stimulation of cell division in lymphocyte cultures. TCL071 is a sterile filtered solution of 1mg per ml PHAM in sterile tissue culture water.

Net weight: 0,01 kg

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[TCL072] HAT supplement contains Hypoxanthine, Aminopterin and Thymidine and is used for the preparation of selection medium for hybridoma. The myeloma cell is deficient in enzyme hypoxanthine-guanine phosphoribosyl transferase (HGPRT) or thymidine kinase (TK), and cannot survive in selection medium containing hypoxanthine, aminopterin and thymidine. TCL072 is a sterile filtered 50X HAT medium supplement containing 680.5 mg/litre Hypoxanthine, 8.8 mg/litre Aminopterin and 193.8 mg/litre Thymidine in Phosphate Buffered Saline.

Net weight: TCL072-100ML: 0,1 kg
TCL072-10X10ML: 0,1 kg
TCL072-5X10ML: 0,05 kg

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[TCL073] The specific action of aminopterin present in the HAT system is to inhibit the de novo DNA synthesis. Hence, it needs to be eliminated once the hybrids are selected and isolated. Aminopterin is diluted by several passages in hypoxanthine-thymidine (HT) supplemented medium for about 2-3 weeks. Once the de novo nucleoside-synthesis pathway has been reinstated, HT supplementation can be discontinued.
TCL073 is a sterile filtered, cell culture tested 50X HT medium supplement containing 680.5 mg/litre Hypoxanthine and 193.8 mg/litre Thymidine in Phosphate Buffered Saline.

Net weight: TCL073-100ML: 0,1 kg
TCL073-10x10ML: 0,1 kg
TCL073-5x10ML: 0,1 kg

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[TCL074] Colcemid, also known as demecolcine, is a synthetic analog of colchicine. It binds to the tubulin protein and obstructs the spindle fibre formation. It effectively arrests cell in metaphase, allowing cell harvest and karyotyping to be performed. Colcemid is used to arrest different types of cells in metaphase like peripheral blood cells, amniotic fluid cells, fibroblasts, bone marrow cells and cells from chorion villus samples, etc. TCL074 is a sterile filtered solution of 10μg per ml of Colcemid is Hank's balanced salt solution.

Net weight: TCL074-100ML: 0,1 kg
TCL074-20ML: 0,02 kg

refrigerated goods (2°C - 8°C)

[TCL077] TCL077 is sterile solution formulated to contain 45% w/v glucose in tissue culture grade water. This solution is sterilized by autoclaving.

Net weight: TCL077-500ML: 0,5 kg
TCL077-100ML: 0,1 kg

[TCL078] Cell Freezing Medium with Glycerol is fully supplemented formulation prepared in Dulbecco’s Modified Eagle Medium. It contains 10% Glycerol and fetal bovine serum. Glycerol acts as a cryoprotectant as it prevents formation of ice crystals and prevents cell damage. It does not contain phenol red. It is ready to use and does not require further addition of any other reagents. This cryopreservation medium can be used for hardy cell lines that are less susceptible to freezing damage.

Net weight: 0,05 kg

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[TCL081] This prroduct is a trypsin-EDTA solution formulated to contain 500BAEE units of porcine trypsin and 180ug EDTA tetrasodium salt per ml in Dulbecco's Phosphate Buffered Saline. It does not contain calcium, magnesium and phenol red.

Net weight: TCL081-6x500ML: 3 kg
TCL081-500ML: 0,5 kg
TCL081-100ML: 0,1 kg

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[TCL083] Giemsa stain is a mixture of methylene blue, eosin, and azure B. It is specific for the phosphate groups of DNA and attaches itself to where there are high amounts of adenine thymine bonding. Giemsa stain is mainly used for staining of peripheral blood smears and specimens obtained from the bone marrow. Giemsa stain is also used in cytogenetics to stain the chromosomes and identify chromosomal aberrations. TCL083 is a sterile filtered concentrated solution of Giemsa stain.

Net weight: TCL083-500ML: 0,5 kg
TCL083-100ML: 0,1 kg

[TCL088] The Glutamine Synthetase Expression Medium (GSEM) with supplement is a system that supports the growth of transfected cells containing the gene for the enzyme Glutamine synthetase. TCL088 is sterile-filtered, 50X liquid Glutamine Synthetase Expression Medium Supplement.

Net weight: 0,1 kg

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[TCL091] In serum supplemented cell cultures, serum serves as a major source of extracellular lipids. In serum-free cell culture systems, lipids are added in the form of lipid supplements or lipid concentrates. These are mixtures of different fatty acids, detergents and lipids that are essential for cell growth and maintenance in serum-free conditions.
TCL091 is a sterile filtered 100X lipid supplement formulated to contain chemically defined lipids and fatty acids.

Net weight: 0,1 kg

refrigerated goods (2°C - 8°C)

[TCL092] Cholesterol supplement is used as an additive in serum free media. It is an important attachment factor for increased plating efficiency. It protects cells from shear stress. TCL092 is a sterile filtered 250X cholesterol supplement.

Net weight: 0,05

refrigerated goods (2°C - 8°C)

[TCL093] Cell Freezing Media are complete, ready to use reagents designed to protect and preserve cells during frozen storage. These media are a convenient and cost effective alternative to in-house freezing media and can be used for a wide variety of mammalian cells. TCL093 contains 10% DMSO and higher concentration of fetal bovine serum. It is ready to use and does not require further addition of any other reagents. This cryopreservation medium can be used for sensitive cell lines that are very susceptible to freezing damage.

Net weight: 0,05 kg

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[TCL097] ITS-L1 supplement is a basal growth factor supplement used to reduce the serum requirements in reduced serum and serum-free cell culture systems.
Insulin is a peptide hormone responsible for uptake, utilization and storage of glucose by cells. Insulin is often used as a medium supplement for cell culture in the range of 1-10 µg/ml depending upon the cell type.

Net weight: 0,01 kg

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[TCL098]
It is chemically defined serum free cell freezing medium designed to freeze the cells grown in serum free and animal component free conditions. This medium ensures high cell viability upon revival from cryo-storage and can also be used for cryopreservation of stem cells and primary cells. This medium is a proprietary formulation.

Net weight: 0,05 kg

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[TCL099] Trypsin is a serine protease derived from porcine pancreas. Ethylenediaminetetraacetic acid (EDTA), a chelating agent is often added to enhance enzymatic activity of trypsin solution. This product contains 0.025% Trypsin and 0.01% EDTA in Dulbecco’s phosphate buffered saline.

Net weight: TCL099-500ML: 0, 5kg
TCL099-5x100ML: 0,5 kg
TCL099-100ML: 0,1 kg

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[TCL109] Gelatin is a protein produced by partial hydrolysis of collagen extracted from boiled bones, hides and skin, connective tissues, organs and some intestines of domesticated animals like cattle, pigs and horses. Gelatin is widely used in cell culture for coating the cell culture plates and flasks to improve the cell attachment for certain types of cells. TCL109 is a sterile filtered 0.5% solution of gelatin from bovine skin prepared in tissue culture grade water. Gelatin is generally used in cell culture applications at a concentration of 0.1%.

Net weight: 0,1 kg