Various cell culture applications are suitable for other animal sera.
APPLICATION:
- Autologous cell culture systems
- Immuno histochemistry
- Immunoblotting
- Immunofluorescence
- Immunoprecipitation
TEST PARAMETER:
- pH
- Osmolality
- Endotoxin
- Total protein
- Hemoglobin
- Mycoplasma
- Sterility

Storage Temperature: -20°C

Various cell culture applications are suitable for other animal sera.
APPLICATION:
- Autologous cell culture systems
- Immuno histochemistry
- Immunoblotting
- Immunofluorescence
- Immunoprecipitation
TEST PARAMETER:
- pH
- Osmolality
- Endotoxin
- Total protein
- Hemoglobin
- Mycoplasma
- Sterility

Storage Temperature: -20°C

Various cell culture applications are suitable for other animal sera.
APPLICATION:
- Autologous cell culture systems
- Immuno histochemistry
- Immunoblotting
- Immunofluorescence
- Immunoprecipitation
TEST PARAMETER:
- pH
- Osmolality
- Endotoxin
- Total protein
- Hemoglobin
- Mycoplasma
- Sterility

Storage Temperature: -20°C

Add heparinized blood 1:1 with PBS and transfer to a centrifuge tube previously filled with lymphocyte separation medium (Pancoll density 1.077 g/ml): pipette carefully to avoid phase mixing!
Panserin 701 is a fully defined, complete ready-to-use serum-free medium for the cultivation of lymphocytes from whole blood.
COMPOSITION:
Based on Iscove's MEM the medium is enriched with additional trace elements, albumin, cholesterol, lipids and vitamins. It contains the mitogen phytohemagglutinin (PHA) for a growth stimulation of lymphocytes.
SUITABILITY AND APPLICATIONS:
Panserin 701 has been developed for the serum-free cultivation of lymphocytes from whole blood. The herbal lectin (PHA) in Panserin 701 stimulates cell division.
APPLICATION:
1. Isolation of lymphocytes from whole blood using density gradient centrifugation.
2. Cultivation and stimulation of lymphocytes.
- Resuspend lymphocytes in Panserin 701 at a cell density of 1 x 10 5 .
- The phytohemagglutinin already contained in Panserin 701 stimulates the cell division of the lymphocytes.
- The incubation time is 48 to 72 hours, depending on the type and origin of the lymphocytes and further use.
- Further cultivation e.g. in Panserin 413. After approx. 14 days, restimulation with Panserin 701 is necessary.

Storage Temperature: -20°C

Panserin 401 is a fully defined complete ready-to-use medium for the serum-free cultivation of a multitude of adherent and non adherent cells.
COMPOSITION:
Based on Iscove's MEM, trace elements, albumin, cholesterol, soya lipids and vitamins were added to the medium. It does not contain any growth or attachment factors.
SUITABILITY:
Panserin 401 is a multi-purpose medium suitable for a variety of cells. As the medium contains no growth factors there is a possibility to investigate the effects of specific growth factors added to the cell culture. Panserin 401 does not contain any attachment factors. With some cell types a pre-treatment of the cell culture vessels with gelatine, collagen, poly-D-lysine or fibronectin may support or enable a culture under serum-free conditions. Please note that a coating may be especially important with low seeding densities. With every adaption to serum-free media, changes of the cells should be taken into consideration. These changes may concern morphology, karyotype, surface markers and so on. Thus cells in serum-free medium may not be identical with those from cultures containing serum in which they originated (selection).
AMONG OTHERS THE FOLLOWING CELLS HAVE BEEN CULTIVATED SUCCESSFULLY::
- Hybridoma
- Lymphocytes
- Macrophages
- Fibroblasts
- Melanocytes
- Carcinoma-cells
- HEK-cells
- HeLa-cells
- CHO-cells
APPLICATION:
Switching from serum-containing medium to Panserin is possible for many cells without special adaptation. For cell lines that do not tolerate such a simple switch to Panserin, we recommend culturing the cells in Panserin supplemented with serum and gradually reducing the serum percentage. This process is supported by higher seeding densities or, for adherent cells, by switching to the next lower serum concentration only after the cells have attached. The vitality state plays a crucial role for a successful transfer of cells into serum-free culture. Therefore, cells must be harvested during the logarithmic growth phase. Based on our experience, cultivation starting from the stationary phase has significantly lower success rates.
For adherent cells, it must be ensured that after potential detachment with trypsin, the enzyme is removed by thorough washing or inhibited by a trypsin inhibitor, as the neutralizing effect present in serum is absent. For very sensitive cells, it may also be useful not only to reduce the serum content but also to slowly dilute the previously used medium with Panserin. Panserin was designed to achieve cell growth without serum. Therefore, the all-round version (Panserin 401) does not contain any additional growth factors. Investigations into the effectiveness of added growth factors thus yield clearer results. For cell lines requiring specific growth factors, these should continue to be added at the usual concentration.

Storage Temperature: +2°C - +8°C

Panserin 293A is a complete ready to use medium for the serum-free cultivation of HEK293 cells (Human Embryonic Kidney) in adherent culture.
COMPOSITION:
Based on DMEM additional trace elements, albumin, cholesterol, soy lipids, vitamins and hormones have been added to the medium.
SUITABILITY AND APPLICATIONS:
Panserin 293A is a particularly enriched medium optimized for the growth of HEK293 cells in adherent culture. HEK293 is frequently used for the expression of recombinant proteins and the proliferation of adenoviruses. Panserin 293A promotes a rapid attachment of the cells and guarantees high cell growth rates.
APPLICATION:
Switching from serum-containing medium to Panserin 293A is often possible without special adaptation. For cell clones that do not tolerate such a simple switch to Panserin, we recommend cultivating the cells in Panserin with serum supplement and gradually reducing the serum content.
This procedure is supported by higher titers during seeding. The vitality status plays a crucial role for the successful transfer of cells into serum-free culture. Therefore, cells must be harvested during the logarithmic growth phase.
From our experience, cultivation from the stationary phase is significantly less likely to succeed. When transferring the cells, it must be ensured that after possible detachment with trypsin, the enzyme is removed by complete washing or inhibited by a trypsin inhibitor, as the neutralizing effect present in serum is omitted.

Storage Temperature: +2°C - +8°C

Our specially developed, proprietary processing methodology for serum enables us to offer a special fetal bovine serum for embryonic stem cells (ES).
ADVANTAGES:
- Reproducible constant growth properties
- Improved cloning efficiency
- More undifferentiated clones
- Permanent strict quality control
- No need for further testing of different batches

Storage Temperature: -20°C

Bovine serum is the blood fraction remaining after the natural coagulation of blood, followed by centrifugation to remove any remaining red blood cells. Fetal bovine serum (FBS) containing an especially high amount of growth factors is the most widely used serum. The production of bovine serum at PAN-Biotech is tightly controlled, from the collection of serum at the slaughterhouse and throughout the whole production cycle which is performed without exception in our own production facilities in Aidenbach, Germany.

Storage Temperature: -20°C

Human platelet lysate (hPL) is a xeno-free alternative to FBS.
Human platelet lysate is a very promising source of bioactive substances with enriched growth factors, not only for in vivo wound healing and tissue repair, but also for the expansion of human cells in vitro .
Human platelet lysate promotes the expansion of human adherent and non-adherent primary cells and other cell lines, including primary mesenchymal stromal cells (MSCs).
Defibrinated hPL is ready to use. No additional Heparin is necessary.
The defibrination is carried out by a method developed by PAN-Biotech.
Hereto no additional enzymes like Thrombin are required.

Storage Temperature: -20°C

Various cell culture applications are suitable for other animal sera.
APPLICATION:
- Autologous cell culture systems
- Immuno histochemistry
- Immunoblotting
- Immunofluorescence
- Immunoprecipitation
TEST PARAMETER:
- pH
- Osmolality
- Endotoxin
- Total protein
- Hemoglobin
- Mycoplasma
- Sterility

Storage Temperature: -20°C

FBS SUPREME: STANDARDIZED FETAL BOVINE SERUM:
Fetal bovine serum is a natural product and may therefore vary from batch to batch. To compensate for these differences between batches, PAN-Biotech developed a unique process which does not affect the biological nature of FBS: Single components naturally present in the serum are enriched in a higher quality thus leading to a more defined product.
Advantages:
- time consuming batch testing not necessary anymore
- differences between batches in the range of +/- 10% only
- constantly on stock
- virus and mycoplasma tested


Storage Temperature: -20°C

Equivalent/Alternative to Biochrom:
100 ml: Biochrom Cat.-no. S 0615

Bovine serum is the blood fraction remaining after the natural coagulation of blood, followed by centrifugation to remove any remaining red blood cells. Fetal bovine serum (FBS) containing an especially high amount of growth factors is the most widely used serum. The production of bovine serum at PAN-Biotech is tightly controlled, from the collection of serum at the slaughterhouse and throughout the whole production cycle which is performed without exception in our own production facilities in Aidenbach, Germany.

Storage Temperature: -20°C

Bovine serum is the blood fraction remaining after the natural coagulation of blood, followed by centrifugation to remove any remaining red blood cells. Fetal bovine serum (FBS) containing an especially high amount of growth factors is the most widely used serum. The production of bovine serum at PAN-Biotech is tightly controlled, from the collection of serum at the slaughterhouse and throughout the whole production cycle which is performed without exception in our own production facilities in Aidenbach, Germany.

Storage Temperature: -20°C

Bovine serum is the blood fraction remaining after the natural coagulation of blood, followed by centrifugation to remove any remaining red blood cells. Fetal bovine serum (FBS) containing an especially high amount of growth factors is the most widely used serum. The production of bovine serum at PAN-Biotech is tightly controlled, from the collection of serum at the slaughterhouse and throughout the whole production cycle which is performed without exception in our own production facilities in Aidenbach, Germany.

Storage Temperature: -20°C

Donor Horse Serum may be preferred to Horse Serum as it can be obtained from a closed herd which makes it more consistent from batch to batch. Furthermore, lower trace metals concentration makes it an ideal supplement for complex media formulations where higher protein concentration is not a factor.

Storage Temperature: -20°C

Various cell culture applications are suitable for other animal sera.
APPLICATION:
- Autologous cell culture systems
- Immuno histochemistry
- Immunoblotting
- Immunofluorescence
- Immunoprecipitation
TEST PARAMETER:
- pH
- Osmolality
- Endotoxin
- Total protein
- Hemoglobin
- Mycoplasma
- Sterility

Storage Temperature: -20°C

Various cell culture applications are suitable for other animal sera.
APPLICATION:
- Autologous cell culture systems
- Immuno histochemistry
- Immunoblotting
- Immunofluorescence
- Immunoprecipitation
TEST PARAMETER:
- pH
- Osmolality
- Endotoxin
- Total protein
- Hemoglobin
- Mycoplasma
- Sterility

Storage Temperature: -20°C

Bovine serum is the blood fraction remaining after the natural coagulation of blood, followed by centrifugation to remove any remaining red blood cells. Fetal bovine serum (FBS) containing an especially high amount of growth factors is the most widely used serum. The production of bovine serum at PAN-Biotech is tightly controlled, from the collection of serum at the slaughterhouse and throughout the whole production cycle which is performed without exception in our own production facilities in Aidenbach, Germany. Bovine Serum is collected from healthy animals, aged between 12 and 36 months.

Storage Temperature: -20°C

Spodopan is a serum-free and protein-free medium for optimized growth of insect cells such as Sf9 and Sf21 ( Spodoptera frugiperda ) in suspension culture.
COMPOSITION:
Spodopan contains amino acids, vitamins, salts, trace elements, lipids and growth promoting factors in a formulation optimized for insect cells. It contains no protein or any other components of human or animal origin, only traces of animal-derived components (< 0.2 % w/v) and hydrolysates (< 0.7 % w/v).
SUITABILITY:
Spodopan is suitable for the cultivation of insect cells and the production of recombinant proteins and viruses (Baculovirus expression vector system, BEVS).

Storage Temperature: +2°C - +8°C

Panserin 413 modified is a serum-free medium optimized for the serum-free in vitro cultivation of lymphocytes and hybridoma cells.
COMPOSITION:
This defined formulation based on RPMI 1640 / DMEM-F12 contains no hydrolysates, only traces of animal-derived components (< 0.6 % w/v) and proteins (< 0.1 % w/v).
SUITABILITY:
Panserin 413 modified is a ready-to-use medium optimized for the serum-free in vitro cultivation of human immune cells isolated from peripheral whole blood. The Panserin 413 modified formulation allows the efficient expansion of T-cells, CIK, LAK and NK cells without the addition of bovine serum or (autologous) human serum. Growth factors for the differentiation or the enrichment of the target cell type from PBMC are not included in this product. Suitable supplements for T-cells can be ordered with the cat.-Nr. P04-413S.
Äquivalent zu: X-Vivo 15, CTS OpTmizer T-Cell Expansion, StemLine T Cell Expansion Medium

Storage Temperature: +2°C - +8°C