PowerStem MSC1 is an easy to use xeno-free medium for in vivo proliferation of human mesenchymal stem cells (hMSC). PowerStem MSC1 supports long-term growth of MSC and preserves their multi-lineage potential. In addition, MSC cultured in PowerStem MSC1 expands faster and shows a significant reduction in hematopoietic cell contamination at early passages compared to serum-based media. To differentiate the proliferated MSC into different cells types the relevant protocols and differentiation factors should be used.
PowerStem MSC1 basal medium is stable for 2 years and PowerStem MSC1 growth supplements are stable for 12 months when properly stored. PowerStem MSC1 complete medium (basal + supplements) is stable for 1 month when stored in the dark at 2 - 8 °C. We do not recommend using the complete medium beyond one month. Do not freeze complete PowerStem MSC1 medium.
COMPOSITION:
PowerStem MSC1 contains salts, amino acids, trace elements, hormones, growth factors, enriched human proteins and lipids in an optimized formulation.
SUITABILITY:
Serum-free cultivation of human mesenchymal stem cells (hMSC) while maintaining the undifferentiated state and multi-lineage potential.

Storage Temperature: +2°C - +8°C/-20°C

Panserin 801 is a serum-free (animal-derived components < 0.5 % w/v) and defined culture medium (total protein < 0.5 % w/v) for the cultivation of keratinocytes. It selectively supports the growth of human keratinocytes and concurrently prevents the overgrowth with fibroblasts.
COMPOSITION:
The kit (P04-710801K) contains chemically defined MCDB-153 as basal medium ( P04-710801B) and the following supplements:
- Epidermal Growth Factor (EGF)
- Insulin
- Hydrocortisone
- Ethanolamine
- Phosphoethanolamine
- Bovine Pituitary Extract (BPE)
Equivalent to: KBM-Gold Basal Medium, Defined Keratinocyte SFM basal medium, StemLine Keratinocyte Medium SFM

Storage Temperature: -20°C

Panserin 801 is a serum-free (animal-derived components < 0.5 % w/v) and defined culture medium (total protein < 0.5 % w/v) for the cultivation of keratinocytes. It selectively supports the growth of human keratinocytes and concurrently prevents the overgrowth with fibroblasts.
COMPOSITION:
The kit (P04-710801K) contains chemically defined MCDB-153 as basal medium ( P04-710801B) and the following supplements:
- Epidermal Growth Factor (EGF)
- Insulin
- Hydrocortisone
- Ethanolamine
- Phosphoethanolamine
- Bovine Pituitary Extract (BPE)
Equivalent to: KBM-Gold Basal Medium, Defined Keratinocyte SFM basal medium, StemLine Keratinocyte Medium SFM

Storage Temperature: +2°C - +8°C

Panserin 293S is a complete ready to use medium for the serum-free cultivation of HEK293 cells (Human Embryonic Kidney) in suspension culture.
COMPOSITION:
Based on DMEM / F12 medium with additional oligopeptides (hydrolysates < 0.02 % w/v), hormones and trace elements to support expression and cell growth. This product contains only traces of protein (< 0.002 % w/v) and animal derived components (< 0.0003 % w/v).
SUITABILITY:
Panserin 293S is a particularly enriched medium optimized for the growth of HEK293 cells in suspension culture. Due to its defined composition the purification of final products (recombinant proteins, viruses) from the cell culture is more convenient and economic. This product can also be applied to adapt adherent HEK293 cells into suspension.

Storage Temperature: +2°C - +8°C

Panserin 293S is a complete ready to use medium for the serum-free cultivation of HEK293 cells (Human Embryonic Kidney) in suspension culture.
COMPOSITION:
Based on DMEM / F12 medium with additional oligopeptides (hydrolysates < 0.02 % w/v), hormones and trace elements to support expression and cell growth. This product contains only traces of protein (< 0.002 % w/v) and animal derived components (< 0.0003 % w/v).
SUITABILITY:
Panserin 293S is a particularly enriched medium optimized for the growth of HEK293 cells in suspension culture. Due to its defined composition the purification of final products (recombinant proteins, viruses) from the cell culture is more convenient and economic. This product can also be applied to adapt adherent HEK293 cells into suspension.

Storage Temperature: +2°C - +8°C

Panserin 293S is a complete ready to use medium for the serum-free cultivation of HEK293 cells (Human Embryonic Kidney) in suspension culture.
COMPOSITION:
Based on DMEM / F12 medium with additional oligopeptides (hydrolysates < 0.02 % w/v), hormones and trace elements to support expression and cell growth. This product contains only traces of protein (< 0.002 % w/v) and animal derived components (< 0.0003 % w/v).
SUITABILITY:
Panserin 293S is a particularly enriched medium optimized for the growth of HEK293 cells in suspension culture. Due to its defined composition the purification of final products (recombinant proteins, viruses) from the cell culture is more convenient and economic. This product can also be applied to adapt adherent HEK293 cells into suspension.

Storage Temperature: +2°C - +8°C

Panserin 411 is a fully defined, complete ready-to-use medium for the serum-free cultivation of a multitude of adherent and non adherent cells which are Insulin-dependent (e.g. CHO-cells).
COMPOSITION:
Based on Iscove's MEM, trace elements, albumin, cholesterol, soy lipids, vitamins and insulin were added to the medium. It does not contain any growth or attachment factors. Protein and animal-derived components < 0.2 % w/v. Hydrolysates < 0.001 % w/v.
SUITABILITY:
Panserin 411 is a multi-purpose medium suitable for a variety of cells. In Panserin 411 adherent as well as non adherent cells can be cultivated. As the medium contains no growth factors there is a possibility to investigate the effects of specific growth factors added to the cell culture. Panserin 411 does not contain any attachment factors. With some cell types a pre-treatment of the cell culture vessels with gelatine, collagen, poly-D-lysine or fibronectin may support or enable a culture under serum-free conditions. Please note that a coating may be especially important with low seeding densities.

Storage Temperature: +2°C - +8°C

Endopan 300 SL kit is provided with serum substitute (Panexin SL-S, P04-0065S) and supplements in separate sterile packing. This will enable the user to prepare a medium for special applications. For example, VEGF, FGF-2, or other components may be omitted from the complete medium for specific experimental settings.
COMPOSITION:
300 SL kit contains the following components in single packing:
- Basal Medium Endopan 300 SL
- Serum substitute Panexin SL-S
- EGF (human recombinant Epidermal Growth Factor)
- FGF-2 (human recombinant basic Fibroblast Growth Factor)
- VEGF (human recombinant Vascular Endothelial Growth Factor)
- Vitamin C (Ascorbic Acid phosphate)
- R3-IGF-1 (human recombinant Insulin-like Growth Factor)
- GA (Gentamycin/Amphotericin)
- Hydrocortisone
- Heparin

Storage Temperature: +2°C - +8°C

Endopan 300 SL is designed for serum-free culture of endothelial cells directly after isolation. This exclusive product is optimized for the maintenance and expansion of endothelial cells under serum-free culture conditions.

Storage Temperature: +2°C - +8°C

Add heparinized blood 1:1 with PBS and transfer to a centrifuge tube previously filled with lymphocyte separation medium (Pancoll density 1.077 g/ml): pipette carefully to avoid phase mixing!
Panserin 701 is a fully defined, complete ready-to-use serum-free medium for the cultivation of lymphocytes from whole blood.
COMPOSITION:
Based on Iscove's MEM the medium is enriched with additional trace elements, albumin, cholesterol, lipids and vitamins. It contains the mitogen phytohemagglutinin (PHA) for a growth stimulation of lymphocytes.
SUITABILITY AND APPLICATIONS:
Panserin 701 has been developed for the serum-free cultivation of lymphocytes from whole blood. The herbal lectin (PHA) in Panserin 701 stimulates cell division.
APPLICATION:
1. Isolation of lymphocytes from whole blood using density gradient centrifugation.
2. Cultivation and stimulation of lymphocytes.
- Resuspend lymphocytes in Panserin 701 at a cell density of 1 x 10 5 .
- The phytohemagglutinin already contained in Panserin 701 stimulates the cell division of the lymphocytes.
- The incubation time is 48 to 72 hours, depending on the type and origin of the lymphocytes and further use.
- Further cultivation e.g. in Panserin 413. After approx. 14 days, restimulation with Panserin 701 is necessary.

Storage Temperature: -20°C

Panserin 401 is a fully defined complete ready-to-use medium for the serum-free cultivation of a multitude of adherent and non adherent cells.
COMPOSITION:
Based on Iscove's MEM, trace elements, albumin, cholesterol, soya lipids and vitamins were added to the medium. It does not contain any growth or attachment factors.
SUITABILITY:
Panserin 401 is a multi-purpose medium suitable for a variety of cells. As the medium contains no growth factors there is a possibility to investigate the effects of specific growth factors added to the cell culture. Panserin 401 does not contain any attachment factors. With some cell types a pre-treatment of the cell culture vessels with gelatine, collagen, poly-D-lysine or fibronectin may support or enable a culture under serum-free conditions. Please note that a coating may be especially important with low seeding densities. With every adaption to serum-free media, changes of the cells should be taken into consideration. These changes may concern morphology, karyotype, surface markers and so on. Thus cells in serum-free medium may not be identical with those from cultures containing serum in which they originated (selection).
AMONG OTHERS THE FOLLOWING CELLS HAVE BEEN CULTIVATED SUCCESSFULLY::
- Hybridoma
- Lymphocytes
- Macrophages
- Fibroblasts
- Melanocytes
- Carcinoma-cells
- HEK-cells
- HeLa-cells
- CHO-cells
APPLICATION:
Switching from serum-containing medium to Panserin is possible for many cells without special adaptation. For cell lines that do not tolerate such a simple switch to Panserin, we recommend culturing the cells in Panserin supplemented with serum and gradually reducing the serum percentage. This process is supported by higher seeding densities or, for adherent cells, by switching to the next lower serum concentration only after the cells have attached. The vitality state plays a crucial role for a successful transfer of cells into serum-free culture. Therefore, cells must be harvested during the logarithmic growth phase. Based on our experience, cultivation starting from the stationary phase has significantly lower success rates.
For adherent cells, it must be ensured that after potential detachment with trypsin, the enzyme is removed by thorough washing or inhibited by a trypsin inhibitor, as the neutralizing effect present in serum is absent. For very sensitive cells, it may also be useful not only to reduce the serum content but also to slowly dilute the previously used medium with Panserin. Panserin was designed to achieve cell growth without serum. Therefore, the all-round version (Panserin 401) does not contain any additional growth factors. Investigations into the effectiveness of added growth factors thus yield clearer results. For cell lines requiring specific growth factors, these should continue to be added at the usual concentration.

Storage Temperature: +2°C - +8°C

Panserin 293A is a complete ready to use medium for the serum-free cultivation of HEK293 cells (Human Embryonic Kidney) in adherent culture.
COMPOSITION:
Based on DMEM additional trace elements, albumin, cholesterol, soy lipids, vitamins and hormones have been added to the medium.
SUITABILITY AND APPLICATIONS:
Panserin 293A is a particularly enriched medium optimized for the growth of HEK293 cells in adherent culture. HEK293 is frequently used for the expression of recombinant proteins and the proliferation of adenoviruses. Panserin 293A promotes a rapid attachment of the cells and guarantees high cell growth rates.
APPLICATION:
Switching from serum-containing medium to Panserin 293A is often possible without special adaptation. For cell clones that do not tolerate such a simple switch to Panserin, we recommend cultivating the cells in Panserin with serum supplement and gradually reducing the serum content.
This procedure is supported by higher titers during seeding. The vitality status plays a crucial role for the successful transfer of cells into serum-free culture. Therefore, cells must be harvested during the logarithmic growth phase.
From our experience, cultivation from the stationary phase is significantly less likely to succeed. When transferring the cells, it must be ensured that after possible detachment with trypsin, the enzyme is removed by complete washing or inhibited by a trypsin inhibitor, as the neutralizing effect present in serum is omitted.

Storage Temperature: +2°C - +8°C

Spodopan is a serum-free and protein-free medium for optimized growth of insect cells such as Sf9 and Sf21 ( Spodoptera frugiperda ) in suspension culture.
COMPOSITION:
Spodopan contains amino acids, vitamins, salts, trace elements, lipids and growth promoting factors in a formulation optimized for insect cells. It contains no protein or any other components of human or animal origin, only traces of animal-derived components (< 0.2 % w/v) and hydrolysates (< 0.7 % w/v).
SUITABILITY:
Spodopan is suitable for the cultivation of insect cells and the production of recombinant proteins and viruses (Baculovirus expression vector system, BEVS).

Storage Temperature: +2°C - +8°C

Panserin 413 modified is a serum-free medium optimized for the serum-free in vitro cultivation of lymphocytes and hybridoma cells.
COMPOSITION:
This defined formulation based on RPMI 1640 / DMEM-F12 contains no hydrolysates, only traces of animal-derived components (< 0.6 % w/v) and proteins (< 0.1 % w/v).
SUITABILITY:
Panserin 413 modified is a ready-to-use medium optimized for the serum-free in vitro cultivation of human immune cells isolated from peripheral whole blood. The Panserin 413 modified formulation allows the efficient expansion of T-cells, CIK, LAK and NK cells without the addition of bovine serum or (autologous) human serum. Growth factors for the differentiation or the enrichment of the target cell type from PBMC are not included in this product. Suitable supplements for T-cells can be ordered with the cat.-Nr. P04-413S.
Äquivalent zu: X-Vivo 15, CTS OpTmizer T-Cell Expansion, StemLine T Cell Expansion Medium

Storage Temperature: +2°C - +8°C