w: 0,5mM EDTA, w: Phenol red

Accutase® enzymes (activity > 500 U/ml) in DPBS w/o Ca/Mg with 0.5 mM EDTA and phenol red

Ready-to-use solution
Gentle and effective cell detachment
Lower contamination risk
No neutralization required
Protection of surface epitopes
Maximum cell viability and enhanced plating efficiency
Alternative to Trypsin

Storage Temperature: -20 °C
Dry Ice Shipment

with L-Glutamine
with 15mM HEPES
with 1,2 g/L NaHCO3
Size 500ml
Storage condition: 2°C - 8°C
Shipping condition: Room Temperature

FGF2-G3 (FGF2-STAB) is a stabilized growth factor that offers a novel way to grow FGF2-dependent cell cultures more efficiently, with fewer media changes. FGF2-G3 retains full biological activity even after five days at 37°C. The stable level of FGF2 in culture allows for a more homogenous, undifferentiated stem cell culture, while saving researchers valuable time and money, as repeated supplementation by FGF2 and every day medium change is not required.

Available as liquid or powder.

*** Dry Ice Shipment necessary for the liquid ***

Defined Bioscience’s FGF2-G3 (bovine), or btFGF2-G3, is a thermostabilized growth factor that offers a novel way to grow FGF2-dependent cell cultures more efficiently, with fewer medium changes. The stability of FGF2-G3 in culture allows for a more homogeneous, undifferentiated stem cell culture, and daily medium change is not required.

Available as liquid or powder.

*** Dry Ice Shipment necessary for the liquid ***

Defined Bioscience’s FGF2 (chicken) growth factor possesses the full native sequence for FGF2 derived from G. gallus, the domestic chicken, produced as an animal-free, carrier-free, and highly pure growth factor. Previous research has shown the value of cross-species homologs in cell culture applications, offering opportunities to use this growth factor in improving workflows across human and non-human cell line culture. Contact us for larger quantities to get a quote.

*** Dry Ice Shipment ***

Defined Bioscience’s FGF2 (tuna) growth factor possesses the full native sequence for FGF2 derived from T. albacares, the yellowfin tuna, produced as an animal-free, carrier-free, and highly pure growth factor. Previous research has shown the value of cross-species homologs in cell culture applications, offering opportunities to use this growth factor in improving workflows across human and non-human cell line culture. Contact us for larger quantities to get a quote.

*** Dry Ice Shipment ***

Defined Bioscience’s FGF2 Screening Kit offers multiple animal-free, recombinantly expressed FGF2 variants for research use. This kit includes four FGF2 variants in easy-to-use aliquots of 10 or 50 µg each, including thermostable and native sequences. Previous research has shown the value of cross-species homologs in cell culture applications, offering opportunities to use these growth factors in improving workflows across human and non-human cell line culture. The kit includes 10- or 50-ug aliquots of each of the following four proteins: FGF2-G3 (human), FGF2-G3 (bovine/porcine), FGF2 (chicken), and FGF2 (tuna).

*** Dry Ice Shipment ***

Defined Bioscience’s HiDef PBS/EDTA is a defined reagent for protein-free adherent cell dissociation, for routine aggregate passaging or single cell suspension. HiDef PBS/EDTA is designed to detach cells from surfaces without impacting cell functionality or surface protein integrity, with applications in stem, tumor, and primary cell culture, among others. HiDef PBS/EDTA can also be used as a cell washing step prior to detachment using other methods, including enzymatic digestion (e.g. trypsinization). HiDef PBS/EDTA is provided as a 1x solution, 125 mL per bottle, and consists of Dulbecco’s Phosphate Buffered Saline (DPBS, without Ca2+ and Mg2+) pH 7.4 with 0.5 mM EDTA. Each lot of HiDef PBS/EDTA is used in combination with HiDef-B8 medium in performance testing in a culture assay evaluating using human iPSCs to confirm appropriate and consistent detachment.

Defined, feeder-free maintenance medium supplement for human iPS cells. HiDef-B8 400X Supplement (1.25 mL), when added to 500 mL basal media (NOT INCLUDED) , is a complete, serum-free, defined formulation designed for the feeder-free maintenance and expansion of human induced pluripotent stem cells (iPSC) in the undifferentiated state.

HiDef-B8 is available in two flexible formats:
​ • HiDef-B8 Complete Medium Kit: Includes a 400X Supplement vial and optimized ​ DMEM/F12 basal medium.
​ • HiDef-B8 400X Supplement Only: Allows the convenience of choosing a compatible basal medium.

Key Features:
​ • Unique formulation enabling flexible feeding schedules, including weekend-free​maintenance.
​ • Compatible with various matrices and passaging methods tailored to specific​applications.
​ • Contains insulin, ascorbic acid-2-phosphate, transferrin, sodium selenite, TGFB3, NRG1, and thermostable FGF2/bFGF, eliminating the need for additional components.

Compatibility and Recommendations:
OLS recommends using its DMEM/F12, Highly Stable (Catalog #990801) for HiDef-B8. The 400X Supplement is also compatible with other DMEM/F12 formulations, supporting PSC maintenance on matrices from multiple manufacturers at coating concentrations as low as 2.4 µg/cm² (e.g., ~1:800 dilution of an 18 mg/mL stock matrix). HiDef-B8 also supports use with defined substrates.

*** Dry Ice Shipment ***

Defined Bioscience’s Ready-CEPT 1000X cocktail is a defined formulation designed for improved stem cell survival and growth during routine passaging, cryopreservation, and freeze-thaw recovery. Ready-CEPT is a single-vial cocktail of chroman-1 (a ROCK inhibitor), emricasan (a pan-caspase inhibitor), polyamines (putrescine, spermine, and spermidine; polycations for cell growth support), and trans-ISRIB (an ISR inhibitor). CEPT was originally developed at the National Institutes of Health (NIH), where it was found to improve single-cell cloning and sorting, as well as improved viability and cell health. These benefits were well-characterized in particular for genetically stable stem cell culture, with benefits in cell passaging, cryoprotection, organoid and embryoid body (EB) formation, and gene editing, with fewer apparent off-target effects compared to other similar products on the market (e.g. Y-27632). Ready-CEPT is provided as a 1000X cocktail in a DMSO/water mixture, all using animal origin-free componentry. Ready-CEPT 1000X is provided in liquid form in 10- and 200-µL volumes, sufficient for 10 and 200 mL of CEPT-supplemented medium, respectively. This supplement can be used with a wide range of formulations, including Defined Bioscience’s fully defined HiDef-B8 medium (Catalog # LSS-204). Each lot of Ready-CEPT is used in combination with HiDef-B8 medium in performance testing in a culture assay evaluating using human iPSCs.

*** Dry Ice Shipment ***

Defined Bioscience’s HiDef-ITS is a defined formulation designed for the routine maintenance and low-density attachment of adherent cells under reduced serum conditions. HiDef-ITS is a combination of insulin (to stimulate glucose uptake), transferrin (to facilitate iron transport), and sodium selenite (a potent antioxidant and common component supplied by serum), previously shown to reduce the amount of FBS needed in the culture of a wide range of cell types, including primary cells, immortalized cell lines, and stem cells. HiDef-ITS should be used with other growth factors, sera, or additives (NOT INCLUDED) depending on your cell culture application.

with stable Glutamin (L-alanyl-L-glutamine)
with 15mM HEPES
with 1,2 g/L NaHCO3
Size 500ml
Storage condition: 2°C - 8°C
Shipping condition: Room Temperature

contains:
1 vial 400X HiDefB8 Supplement
500ml DMEM/F12, Highly Stable

Product Description:
HiDefB8 Supplement
Defined, feeder-free maintenance medium supplement for human iPS cells. HiDef-B8 400X Supplement (1.25 mL), when added to 500 mL DMEM/F12, highly stable, is a complete, serum-free, defined formulation designed for the feeder-free maintenance and expansion of human induced pluripotent stem cells (iPSC) in the undifferentiated state.

Key Features:
​ • Unique formulation enabling flexible feeding schedules, including weekend-free​maintenance.
​ • Compatible with various matrices and passaging methods tailored to specific​applications.
​ • Contains insulin, ascorbic acid-2-phosphate, transferrin, sodium selenite, TGFB3, NRG1, and thermostable FGF2/bFGF, eliminating the need for additional components.

Storage conditions:
HiDef B8 Supplement *** -20°C ***
DMEM/F12 Medium *** 2-8°C ***

Shipping conditions:
HiDef B8 Supplement *** Dry Ice Shipment ***
DMEM/F12 Medium *** Room Temperature ***

HiDef N-2 is a defined formulation designed for the culture and growth of neurons, neural progenitor cells (NPCs), stem cells, and neuroblastoma (NB) cells, among other cell types, as well as differentiation of embryonic and induced pluripotent stem cells in neural and pancreatic lineages. HiDef N-2 is a combination of insulin (to stimulate glucose uptake), transferrin (to facilitate iron transport), sodium selenite (a potent antioxidant and common medium component), putrescine (for cell growth support), and progesterone (for promoting growth and neuronal differentiation and stimulation). N-2 has been historically shown to improve survival and expression of neuronal cells, including neurons in primary culture, and serves as an effective substitute for the N-1 Bottenstein formulation.

*** Dry Ice Shipment ***

Cleaning agents for anaerobic jars

Concentration 0.2 mg/mL
Applications ELISA, WB
Host Species Goat
Species Reactivity Mouse
Physical State Liquid
Suggested Dilution Range 1:1000 – 1:3000 for ELISA and Western blotting
Purity The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.
Storage Buffer 0.01 M Tris-HCl, 0.25 M NaCl, 50% glycerol, 5 mg/ml BSA, 0.01% Sodium Azide, pH 8.0.
Preservative 0.01% Sodium Azide
Storage Instructions Store at -20°C. Stable for one year after shipment.
Stabilizer 5 mg/ml BSA
Clonality Polyclonal
RRID AB_2890887
Note This product is for research use only,not for diagnostic or therapeutic use.

Concentration 0.2 mg/mL
Applications ELISA, WB
Host Species Goat
Species Reactivity Mouse
Physical State Liquid
Suggested Dilution Range 1:1000 – 1:3000 for ELISA and Western blotting
Purity The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.
Storage Buffer 0.01 M Tris-HCl, 0.25 M NaCl, 50% glycerol, 5 mg/ml BSA, 0.01% Sodium Azide, pH 8.0.
Preservative 0.01% Sodium Azide
Storage Instructions Store at -20°C. Stable for one year after shipment.
Stabilizer 5 mg/ml BSA
Clonality Polyclonal
RRID AB_2890887
Note This product is for research use only,not for diagnostic or therapeutic use.

Concentration 0.2 mg/mL
Applications ELISA, WB
Host Species Goat
Species Reactivity Mouse
Physical State Liquid
Suggested Dilution Range 1:1000 – 1:3000 for ELISA and Western blotting
Purity The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.
Storage Buffer 0.01 M Tris-HCl, 0.25 M NaCl, 50% glycerol, 5 mg/ml BSA, 0.01% Sodium Azide, pH 8.0.
Preservative 0.01% Sodium Azide
Storage Instructions Store at -20°C. Stable for one year after shipment.
Stabilizer 5 mg/ml BSA
Clonality Polyclonal
RRID AB_2890887
Note This product is for research use only,not for diagnostic or therapeutic use.

A precision disposable plastic hemocytometer, developed to solve the problems of conventional Glass hemocytometer
(50 Slides/box, 100 Tests, 100μm)

Customs Tariff Code: 3926 9097
Country of Origin: South Korea

A precision disposable plastic hemocytometer, developed to solve the problems of conventional Glass hemocytometer
(50 Slides/box, 100 Tests, 100μm)

Customs Tariff Code: 3926 9097
Country of Origin: South Korea