Panserin 701, Serum-free medium for Lymphocytes, w: L-Glutamine, w: PHA-L
Add heparinized blood 1:1 with PBS and transfer to a centrifuge tube previously filled with lymphocyte separation medium (Pancoll density 1.077 g/ml): pipette carefully to avoid phase mixing!
Panserin 701 is a fully defined, complete ready-to-use serum-free medium for the cultivation of lymphocytes from whole blood.
COMPOSITION:
Based on Iscove's MEM the medium is enriched with additional trace elements, albumin, cholesterol, lipids and vitamins. It contains the mitogen phytohemagglutinin (PHA) for a growth stimulation of lymphocytes.
SUITABILITY AND APPLICATIONS:
Panserin 701 has been developed for the serum-free cultivation of lymphocytes from whole blood. The herbal lectin (PHA) in Panserin 701 stimulates cell division.
APPLICATION:
1. Isolation of lymphocytes from whole blood using density gradient centrifugation.
2. Cultivation and stimulation of lymphocytes.
- Resuspend lymphocytes in Panserin 701 at a cell density of 1 x 10 5 .
- The phytohemagglutinin already contained in Panserin 701 stimulates the cell division of the lymphocytes.
- The incubation time is 48 to 72 hours, depending on the type and origin of the lymphocytes and further use.
- Further cultivation e.g. in Panserin 413. After approx. 14 days, restimulation with Panserin 701 is necessary.
Storage Temperature: -20°C
Interne Referenz: P04-710701
Specifications
| Size | 100 ml or 500 ml |