[AT237] AT237 is Minimum Essential Medium Eagle/ Nutrient Mixture F-12 Ham (MEM /F12 1:1 Mixture) with 15mM HEPES buffer, 2.5mM L-Glutamine.It does not contain
sodium bicarbonate.

Price for 20L on request.

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[AT238] AT238 is Minimum Essential Medium Eagle with Earle's balanced salts, L-glutamine and non-essential amino acids. It is modified for use with suspension cells, hence does not contain calcium chloride and magnesium sulphate. The medium was developed to meet the specific nutritional requirements of certain subtypes of Hela cells and normal mammalian fibroblasts.

Price for 20L, 5L on request.

refrigerated goods (2°C - 8°C)

[AT239] Dulbecco's Modified Eagle Medium /Nutrient Mixture F12 Ham (DMEM/F12, 1:1mixture) was originally formulated for rat neuroblastoma cells and MDCK cells. DMEM/ Nutrient Mixture F-12 Ham with Lglutamine. It does not contain HEPES buffer, phenol red and trace elements.

Price for 20L, 5L on request.

refrigerated goods (2°C - 8°C)

[AT241] DMEM is a modification of Basal Medium Eagle (BME) that contains four fold concentration of amino acids and vitamins. DMEM with L-glutamine, 4.5gms per litre glucose, sodium pyruvate and 25mM HEPES buffer.

Price for 50L, 20L on request.

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[AT242A] Basal Medium Eagle (BME) w/ Earle’s salts w/o L-Glutamine and Sodium bicarbonate

Price for 20L, 5L on request.

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[AT243] Dulbecco's Modified Eagle Medium /Nutrient Mixture F12 Ham (DMEM/F12, 1:1mixture) was originally formulated for rat neuroblastoma cells and MDCK cells.AT243 is DMEM/ Nutrient Mixture F-12 Ham with Lglutamine, 2mg/L Riboflavin and 55mg/L Sodium pyruvate. It does not contain HEPES buffer, trace elements and Pyridoxal hydrochloride

Price for 50L, 20L, 5L on request.

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[AT253] AT253 is alpha modification of Minimum Essential Medium Eagle with L-glutamine, non-essential amino acids, deoxyribonulceosides and ribonucleosides. It
does not contain phenol red. The medium was developed to meet the specific nutritional requirements of certain subtypes of Hela cells and normal mammalian fibroblasts.

Price for 20L, 5L on request.

refrigerated goods (2°C - 8°C)

[AT261A] Iscove's Modified Dulbecco's Medium is an enriched modification of Dulbecco's Modified Eagle's Medium wherein serum can be partially or totally replaced by chemically defined substances. Iscove's Modified Dulbecco's Medium does not contain L-glutamine, HEPES buffer and phenol red.

Price for 20L on request.

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[AT262F] DMEM is a modification of Basal Medium Eagle (BME) that contains four fold concentration of amino acids and vitamins.AT262F is Dulbecco's Modified Eagle Medium with
4.5gm glucose per litre, L-Glutamine and sodium pyruvate. It does not contain phenol red, folic acid and sodium bicarbonate.

Price for 50L, 20L on request.

refrigerated goods (2°C - 8°C)

[AT285] Minimum Essential Medium Eagle (MEM) (Alpha Modification) w/ L-Glutamine
w/o Sodium pyruvate, Ribonucleosides and deoxyribonucleosides

Price for 20L, 5L on request.

refrigerated goods (2°C - 8°C)

[AT287A] DMEM is a modification of Basal Medium Eagle (BME) that contains four fold concentration of amino acids and vitamins.DMEM with L-glutamine, 4.5gms per litre glucose, calcium chloride, sodium pyruvate and HEPES buffer.

Price for 50L, 20L, 5L on request.

refrigerated goods (2°C - 8°C)

[AT290] AT290 is Nutrient Mixture F-12 Ham with 10mM HEPES and L-glutamine. This medium supports growth of cells of rodent origin. It also allows cloning of myeloma and hybridoma cells and it is medium of choice for clonal toxicity assay using CHO cells.

Price for 20L, 5L on request.

refrigerated goods (2°C - 8°C)

[AT293A] DMEM is a modification of Basal Medium Eagle (BME) that contains four fold concentration of amino acids and vitamins.DMEM with L-glutamine, 4.5gms per litre glucose, calcium chloride, sodium bicarbonate, sodium pyruvate and HEPES buffer.

Price for 50L, 20L, 5L on request.

refrigerated goods (2°C - 8°C)

[AT300] AT300 is alpha modification of Minimum Essential Medium Eagle with L-glutamine. It does not contain deoxyribonucleosides, ribonucleosides and phenol red and sodium bicarbonate. The medium was developed to meet the specific nutritional requirements of certain subtypes of Hela cells and normal mammalian fibroblasts.

Price for 20L, 5L on request.

refrigerated goods (2°C - 8°C)

[CCK001] Melanocytes are the cells that produce melanin and are located in the bottom layer of epidermis of skin, middle layer of the eye, the inner ear, meninges, bones and heart. EZSepTM Melanocyte Isolation Kit is designed for easy isolation of Melanocytes from skin grafts and helps to obtain a very high yield of cells. EZSepTM Melanocyte Isolation Kit is based on the classical method of enzymatic dissociation of the skin grafts at the epidermal-dermal interface and isolation of the cells from the dissociated tissue.

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[CCK003] The EZcountTM MTT Cell Assay kit is designed for determination of cell viability and cell proliferation and/or effect of cytotoxic agent. This kit is based on the quantitative measurement of extracellular reduction of the yellow colored water soluble Tetrazolium dye 3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyl tetrazolium bromide(MTT) to insoluble formazan crystals by metabolically active cells.

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[CCK004] The EZBlue™ Cell Assay Kit is based on reduction of the water soluble, redox dye EZBlue™. The dye itself is dark blue in color and has intrinsic fluorescence. The fluorescence changes when EZBlue™ is reduced by metabolically active cells to the pink resorufin product. The spectral properties of resorufin allows the molecule to be detected using either absorbance or fluorescence.

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[CCK006] EZkillTM Mycoplasma Elimination Kit is an antibiotic based elimination kit designed to eliminate mycoplasma from cell culture. Mycoplasma contamination of cell culture is a major problem in both research and industrial production. Ideally it is recommended to discard the contaminated culture after autoclaving. Only in cases where cell line is very important and non-replaceable, treatment for elimination should be considered. Antibiotic based method is the most common and effective method with no marked cytotoxicity.

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[CCK007] The EZdetectTM DAPI stain kit has been designed for detection of mycoplasma based on rapid uptake of the fluorescent stain DAPI by cellular DNA. DAPI selectively binds minor grooves of the DNA. The excitation maximum of dsDNA-bound DAPI is 358nm and emission maximum is 461nm. Mycoplasma staining with DAPI appears as a fine particulate or filamentous staining over the cytoplasm at 100X magnification. Nuclei of the cells are also brightly stained by this method and thereby act as endogenous positive control for the staining procedure.

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[CCK008] The EZdetectTM Hoechst stain kit has been designed for detection of mycoplasma based on rapid uptake of the fluorescent stain Hoechst by cellular DNA. It selectively binds minor grooves of the DNA and hence allows for efficient staining of the nuclei. Hoechst stain can be excited either with a xenon mercury arc lamp or a UV laser and is detected through a blue filter. Mycoplasma staining with Hoechst appears as a fine particulate or filamentous staining over the cytoplasm at 100X magnification. Nuclei of the cells are also brightly stained by this method and thereby act as endogenous positive control for the staining procedure.

refrigerated goods (2°C - 8°C)