[CCK009] Mycoplasmas are the smallest known, simple prokaryotes, constituting one of the most widely spread contaminants found in cell culture. This kit has been designed for detection of mycoplasma based on amplification of spacer region between 16S and 23S rRNA genomic DNA sequence. This sequence is highly conserved between various mycoplasma species. This allows for detection of commonly encountered cell culture contaminants. Eukaryotic DNA is not amplified. A successful PCR reaction is indicated by a distinct band of 350-400bp derived from the internal control DNA on the agarose gel.

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[CCK011] 3T3-L1 cells are pre-adipocytic in nature. Preadipocytes committed to adipogenesis have to withdraw from the cell cycle before undergoing adipose conversion. Differentiation is achieved by culturing cells progressively in Differentiation medium 1 and Differentiation medium 2. Adipogenesis can be visualized or quantified by the ready–to-use Oil-red-O staining regents included in the kit.

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[CCK013] Adipogenesis is the process of development of adipocytes from pre-adipocytes or reengineered fibroblasts or mesenchymal stem cells and is achieved through proliferation and differentiation of preadipocytes. Adipocytes or adipogenesis can be visualized or quantified by the ready-to-use staining reagents supplied in the kit.

[CCK015] The EZcountTM XTT Cell Assay kit is designed for determination of cell viability and cell proliferation and / or effect of cytotoxic agent. This kit is based on quantitative measurement of extracellular reduction of XTT to water soluble orange coloured formazan derivative by metabolically active cells.

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[CCK022] Mycoplasmas are the smallest known, simple prokaryotes, constituting one of the most widely spread contaminants found in cell culture. PCR based detection of mycoplasma is one of the most convenient method. This kit has been designed for detection of mycoplasma based on amplification of 16S rRNA genomic DNA sequence. This sequence is highly conserved between various mycoplasma species. Eukaryotic DNA is not amplified. A successful PCR reaction is indicated by a distinct band of 502 - 520bp derived from the internal control DNA on the agarose gel.

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[CCK023] Melondialdehyde(MDA) is one of such TBARS used for estimation of lipid peroxidation. This assay is based on the reaction of MDA with a chromogenic agent thiobarbituric acid(TBA) at high temperature and acidic conditions to form MDA-TBA adduct(1:2). This complex has absorption maximum at 532nm and can be measured spectrophotometrically between 530 - 540nm. Absorbance is directly proportional to the TBARS concentration in the sample.

REFRIGERATED GOODS (2°C - 8°C)

[CCK028] EZAssayTM GST Activity Estimation kit is simple and convenient tool for sensitive and reproducible detection and quantitation of GST activity in an array of samples like serum, cell lysates and tissue homogenates. This assay is based on the GST-catalyzed reaction between reduced glutathione and the GST substrate 1-chloro-2,4-dinitrobeneze(CDNB). CDNB can react with the broadest range of GST isoenzymes. The conjugation of glutathione to the CDNB substrate results in an increase in absorbance at 340nm.

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[CCK029] Chondrocytes are mature cells found in cartilage. They make up the cellular matrix of cartilage. EZStainTM Chondrocyte Staining Kit is a ready-to-use kit developed for detection of chondrocytes by staining the proteoglycan produced by them.

[CCK030] Osteogenesis is a process of development of new bone mass from undifferentiated tissues. EZstainTM Osteocyte Staining Kit is a ready-touse kit developed for visualization of osteocytes or osteogenesis in cultured cells or tissue sections.

[CCK031] The EZcountTM BrdU Cell Assay kit is designed for determination of cell proliferation and / or effect ofcytotoxic agent. When cells are cultured with labeling medium containing Pyrimidine analogue 5-bromo-2’-deoxyuridine(BrdU), BrdU gets incorporated in the DNA of proliferating cells in place of thymidine. BrdU labelled proliferating cells can be detected using Anti-BrdU antibody. The labeling medium is removed, and cells are fixed and DNA is denatured using fixation/denaturation solution to make BrdU accessible to the Anti-BrdU-HRP antibody. HRP substrate TMB is added for color development. The magnitude of the absorbance for the developed color directly correlates to the quantity of BrdU incorporated into cells, which is a direct indication of cell proliferation.

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[CCK032] The EZcountTM WST-1 Cell Assay kit is designed for determination of cell viability, cell proliferation and cytotoxicity. This kit is based on the quantitative measurement of extracellular reduction of the water soluble tetrazolium dye WST-1 [2-(4-Iodophenyl)-3-4 -nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium sodium salt] to a water-soluble coloured formazan product by metabolically active cells.

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[CCK034] The EZcountTM Neutral Red Cell Assay Kit is designed for determination of cell viability and cell proliferation and/or effect of cytotoxic agent. This kit is based on the quantitative measurement of uptake of neutral red by cellular lysosomes. Neutral red is a weak cationic dye that readily penetrates the cell membrane by non-ionic diffusion.

[CCK035] Alkaline phosphatase(ALP) is a group of isoenzymes that hydrolyze phosphate esters and generate inorganic phosphate at an alkaline pH. CCK035 is a simple colorimetric kit designed to detect the presenceof alkaline phosphatase activity.

refrigerated goods (2°C - 8°C)

[CCK036] Lactate dehydrogenase(LDH) is a cytoplasmic enzyme present in all mammalian cells. Plasma membrane of live cells is impermeable to LDH. However it is rapidly released into the culture medium when plasma membrane is damaged. CCK036 has been designed for rapid determination of cell cytotoxicity using an enzyme coupled two step reaction.

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[CCK037] L-glutamate is most abundant intracellular amino acid whereas L-glutamine is the most abundant extracellular amino acid. Estimation of L-glutamate and L-glutamine levels in the culture medium is thus indicative of the cellular metabolism and health of the cells. CCK037 is designed for estimation of concentration of these amino acids in cell culture samples such as cell lysates and spent media.

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[CCK044] Microscopy Slide Set for Mammalian Cell lines
Contains a set of slides of five cell lines:
a) BHK-21
b) CHO
c) Vero
d) HEK-293
e) Sp2/O

[CCK049] HiDiffTM Osteogenesis Assay Kit has been developed for in vitro differentiation of MC3T3-E1 cells (preosteoblasts) into mature osteoblasts, determined by staining for mineralization and visualization.

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[CCK051] The EZcountTM ATP Cell Assay Kit is designed for determination of cell viability and cell proliferation and/or effect of cytotoxic agents. The kit is based on the generation of luminescence as ATP catalyzes the reaction between D-luciferin and enzyme luciferase. ATP is ubiquitously present in all viable, metabolically active cells. On the other hand, cell death is characterized by a significant decrease in the ATP level.

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[CCK052] The EZcount™ Sulphorhodamine B cell assay kit is designed for determination of cell viability and cell proliferation and/or effect of cytotoxic agents. This kit is based on the quantitative measurement of cellular protein.

[CCK053] The EZcountTM MTS Cell Assay kit is designed for determination of cell viability and cell proliferation and/or effect of cytotoxic agent. This kit is based on the quantitative measurement of extracellular reduction of the water soluble tetrazolium dye MTS [2-(4-Iodophenyl)-3-4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium sodium salt] to water soluble coloured formazan product by metabolically active cells.

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