iPSC Medium HiDef-B8 – Feeder-Free and Weekend-Free

B8 Medium for Feeder-Free, Weekend-Free iPSC Culture

Enhanced growth of stem cells

• Animal-free, serum-free pluripotency medium
• Maintains iPSCs pluripotent for more than 50 passages without karyotypic abnormalities
• No further components required
• Straightforward medium reconstitution by adding supplement to DMEM
  
  

* Supplement Only: A convenient way to reconstitute HiDef-B8 Complete Medium whenever DMEM/F12 is already available in your laboratory. Just add 1.25 mL (1 vial) of HiDef-B8 400X Supplement to one bottle (500 ml) of DMEM/F12.

HiDef-B8 Complete Stem Cell Growth Medium

HiDef-B8 is a complete, serum-free, defined formulation designed for the feeder-free maintenance and expansion of human pluripotent stem cells (PSCs) in an undifferentiated state. The HiDef-B8 medium is a unique formulation that offers the modern conveniences of a flexible feeding schedule (including weekend-free maintenance) and the ability to choose the matrices and passaging methods that best suit specific applications.

For reconstituting the HiDef-B8 Complete Medium, easily add 1,25 ml of HiDef-B8 400X supplement to 500 mL of DMEM/F12 and it is ready to use.

DMEM/F12 Basal Medium, highly stable

DMEM/F12 is a fully defined basal medium for use in the culture of a wide range of mammalian cells.

DMEM/F12 is a 1:1 mixture of DMEM and Ham’s F-12, providing an optimized environment for a broad range of cell types. Our DMEM/F12 is buffered with HEPES and sodium bicarbonate, offering a comprehensive buffering system which helps to maintain pH stability and minimize the effect of CO2 fluctuations in standard incubator conditions for mammalian cell culture.

Additionally, this formulation includes L-alanyl-L-glutamine in place of glutamine, which enhances cell metabolism and reduces the risk of cell death, while also improving medium stability and cell culture consistency.